Prion protein protocols / Andrew F. Hill, editor.

Format:

Book

Contributor:

Hill, A. F. (Andrew F.)

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 459.

Methods in molecular biology, 1064-3745 ; 459

Language:

English

Subjects (All):

Prions--Laboratory manuals.

Prions.

Prion diseases--Laboratory manuals.

Prion diseases.

Prions--pathogenicity.

Prions--isolation & purification.

Medical Subjects:

Prions--pathogenicity.

Prions--isolation & purification.

Genre:

Laboratory manuals.

Physical Description:

xiii, 280 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana ; London : Springer [distributor], [2008]

Summary:

While much information has been gained over the last two decades regarding the molecular nature of prion proteins, only recently have several research groups begun to cast light on turning the normal prion protein into its aberrant, infectious form. In Prion Protein Protocols, an international team of experts provide an up-to-date collection of current methods in this unique area of neuroscience. The chapters contained in this volume, both protocols and useful review chapters, feature topics such as the cell biology of prions, techniques and approaches for studying prion infection in cultured cells, how these systems can be used as a rapid bioassay, and prion protein misfolding. As a volume in the highly successful Methods in Molecular Biology series, the chapters include readily reproducible, step-by-step laboratory protocols, lists of the necessary materials and reagents, and Notes sections, which provide tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Prion Protein Protocols is an ideal collection for researchers investigating the growing field of mammalian prion disease.

Contents:

Cell culture models to unravel prion protein function and aberrancies in prion diseases / Katarina Bedecs

Investigation of PrPc metabolism and function in live cells : methods for studying individual cells and cell populations / Cathryn L. Haigh and David R. Brown

Immunodetection of PrPsc using western and slot blotting techniques / Hanny Gyllberg and Kajsa Löfgren

Assaying prions in cell culture : the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA) / Sukhvir P. Mahal ... [et al.]

Generation of cell lines propagating infectious prions and the isolation and characterization of cell-derived exosomes / Laura J. Vella and Andrew F. Hill

Neurotoxicity of prion peptides on cultured cerebellar neurons / Giuseppe D. Ciccotosto, Roberto Cappai, and Anthony R. White

Understanding the nature of prion diseases using cell-free assays / Victoria A. Lawson

Methods for conversion of prion protein into amyloid fibrils / Leonid Breydo, Natallia Makarava, and Ilia V. Baskakov

Amplification of purified prions in vitro / Surachai Supattapone, Nathan R. Deleault, and Judy R. Rees

Expression and purification of full-length recombinant PrP of high purity / Natallia Makarava and Ilia V. Baskakov

Analysis of PrP conformation using circular dichroism / Sen Han and Andrew F. Hill

Effect of copper on the de novo generation of prion protein expressed in Pichia pastoris / Carina Treiber

Biophysical investigations of the prion protein using electron paramagnetic resonance / Simon C. Drew and Kevin J. Barnham

Molecular diagnosis of human prion disease / Jonathan D.F. Wadsworth ... [et al.]

Analysis of endogenous PrPc processing in neuronal and non-neuronal cell lines / Victoria Lewis and Steven J. Collins

Molecular typing of PrPres in human sporadic CJD brain tissue / Victoria Lewis ... [et al.]

Transgenic mouse models of prion diseases / Glenn C. Telling

Quantitiative bioassay of surface-bound prion infectivity / Victoria A. Lawson.

Notes:

Includes bibliographical references and index.

ISBN:

9781588298973

1588298973

OCLC:

213383803