Stem cell culture / edited by Jennie P. Mather.

Format:

Book

Contributor:

Mather, Jennie P., 1948-

Series:

Methods in cell biology ; v. 86.

Methods in cell biology ; v. 86

Language:

English

Subjects (All):

Stem cells.

Stem cells--Research.

Cell culture.

Stem Cells.

Cell Culture Techniques.

Biomedical Research.

Medical Subjects:

Stem Cells.

Cell Culture Techniques.

Biomedical Research.

Physical Description:

xix, 386 pages, 16 unnumbered pages of plates : illustrations (some color) ; 24 cm.

Edition:

First edition.

Place of Publication:

London ; Burlington, MA : Elsevier/Academic Press, 2008.

Summary:

Interest in the therapeutic value of embryonic, fetal and adult stem cell types is rapidly expanding throughout the scientific community. The field of stem cell culture is made more challenging, and thus more exciting, by the special properties of these fascinating cells which can both self-renew and differentiate into many types of cells in the adult. The first half of this century should see an explosion of therapeutic applications of stem cells which will grow from the cells and techniques described in this book.

Stem Cell Culture provides a comprehensive resource for researchers in the fields of embryonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentiation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Chapters focus on the practical do's and don'ts of isolating and culturing these cell types, and use illustrative data or diagrams that allow the reader to confidently apply techniques and make this a standard reference.

Key Features: Compares and contrasts a broad range of species and stem cell types, Describes techniques in stem cell research, and goes beyond just protocols by presenting methods within perspective of recent literature reviews, Delineates critical steps and potential pitfalls for each method, provided by at-the-bench authors for expert information, Covers specific procedures in dealing with Human Embryonic Stem Cells, but also emphasizes other sources of potentially therapeutic stem cells such as amniotic fluid, adult muscle and adipose tissue, and umbilical cord.

Contents:

1 Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions / Eric Chiao, Muriel Kmet, Barry Behr, Julie Baker

II Methods 2

2 Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder-Free Conditions / Andre Choo, Ang Sheu Ngo, Vanessa Ding, Steve Oh, Lim Sai Kiang

II Methods and Materials 17

III Results 21

3 Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation / Steven D. Sheridan, Sonia Gil, Matthew Wilgo, Aldo Pitt

III Methods 32

4 Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal / Hiroshi Kubota, Ralph L. Brinster

III Methods 67

5 Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability / Laura Perin, Sargis Sedrakyan, Stafano Da Sacco, Roger De Filippo

II Amniotic Fluid Cell Composition and Stem Cells 87

III In Vitro Potential of Amniotic Fluid Stem Cells 91

IV Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells 94

6 Method to Isolate Mesenchymal-Like Cells from Wharton's Jelly of Umbilical Cord / Kiran Seshareddy, Deryl Troyer, Mark L. Weiss

III Methods 103

V UCMSCs and Culture Characteristics 115

7 Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs) / J. Ennis, R. Sarugaser, A. Gomez, Dolores Baksh, John E. Davies

II Methods 123

8 Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance / Eliane Wauthier, Eva Schmelzer, William Turner, Lili Zhang, Ed LeCluyse, Joseph Ruiz, Rachael Turner, M. E. Furth, Hiroshi Kubota, Oswaldo Lozoya, Claire Barbier, Randall McClelland, Hsin-lei Yao, Nicholas Moss, Andrew Bruce, John Ludlow, L. M. Reid

II Epithelial-Mesenchymal Relationship 139

III Stem Cells and Lineage Biology 140

IV Liver Processing 150

V Fractionation of Liver Cell Subpopulations 162

VI Feeder Cells 170

VII Extracellular Matrix 174

VIII Culture Media 176

IX Monolayer Cultures of Cells 180

X Three-Dimensional Systems 185

Appendix 1 Nomenclature, Glossary, and Abbreviations 195

Appendix 2 Details on the Preparation of Buffers/Reagents 207

Appendix 3 Commercial Sources of Reagents 211

Appendix 4 Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells 212

9 Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo / Ronghao Li, Jennie P. Mather

III Methods 229

10 Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells / Deryk Loo, Claude Beltejar, Jeff Hooley, Xiaolin Xu

III Methods 245

11 Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue / Susan H. Bernacki, Michelle E. Wall, Elizabeth G. Loboa

II Isolation of hMSCs from Trabecular Bone 259

III Isolation of hMSCs from Adipose Tissue 263

IV Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs 265

V Surface Marker Characterization of hMSCs 267

VI hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways 270

12 Culture of Mesenchymal Stem/Progenitor Cells in Adhesion-Independent Conditions / Dolores Baksh, John E. Davies

II Suspension Culture of Bone Marrow-Derived MPCs 280

III Analysis of Suspension Culture 283

IV Mechanism of MPC Expansion in Suspension 288

13 Purification and Long-Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes / Mihaela Crisan, Bridget Deasy, Manuela Gavina, Bo Zheng, Johnny Huard, Lorenza Lazzari, Bruno Peault

II Materials and Methods: Cell Analysis and Purification by Flow Cytometry 298

III Materials and Methods: Long-Term Culture of Human Blood Vessel-Associated Progenitor Cells 301

IV Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes 303

V Materials and Methods: Genotypic and Phenotypic Analyses of Long-Term Cultured Cells 304

14 Isolation and Culture of Colon Cancer Stem Cells / Patrizia Cammareri, Ylenia Lombardo, Maria Giovanna Francipane, Sebastino Bonventre, Matilde Todaro, Giorgio Stassi

II Identification of CSCs in Colon Cancer Tissue 314

III Isolation and Propagation of Colon CSCs 315

15 Isolation and Establishment of Human Tumor Stem Cells / Penelope E. Roberts

III Methods 327

IV Results 336

16 Stem Cells from Cartilaginous and Bony Fish / David W. Barnes, Angela Parton, Mitsuru Tomana Jae-Ho Hwang, Anne Czechanski, Lanchun Fan, Paul Collodi

I Introduction to Models and Uses 344

II Embryonal Stem Cells 348

III Tissue-Specific Stem Cells 353

IV Outlook and Future Contributions 361.

Notes:

Includes bibliographical references and index.

ISBN:

9780123738769

0123738768

OCLC:

213839463