Proteomics in practice : a guide to successful experimental design / edited by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker.

Format:

Book

Contributor:

Westermeier, Reiner.

Naven, Tom.

Höpker, Hans-Rudolf.

Language:

English

Subjects (All):

Proteomics--Handbooks, manuals, etc.

Proteomics.

Proteins--Analysis.

Proteins.

Molecular biology.

Proteome--analysis.

Proteomics--methods.

Medical Subjects:

Proteome--analysis.

Proteomics--methods.

Genre:

Handbooks and manuals.

Physical Description:

xx, 482 pages : illustrations (some color) ; 25 cm

Edition:

Second completely revised edition.

Place of Publication:

Weinheim : Wiley-VCH, [2008]

Summary:

A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries. The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third section is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further detail.

Contents:

Preface

Foreword

Abbreviations, symbols, units

Introduction

pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions

pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search

pt. III. Trouble shooting

Glossary of terms.

Notes:

Includes bibliographical references and index.

ISBN:

9783527319411

3527319417

OCLC:

195752789