Histological and histochemical methods : theory and practice / J. A. Kiernan.

Format:

Book

Author/Creator:

Kiernan, J. A. (John Alan)

Language:

English

Subjects (All):

Histology--Technique.

Histology.

Histochemistry--Technique.

Histochemistry.

Histological Techniques.

Medical Subjects:

Histological Techniques.

Physical Description:

xvi, 606 pages : illustrations ; 25 cm

Edition:

Fourth edition.

Place of Publication:

Bloxham : Scion, [2008]

Summary:

Every chapter in this new edition has been comprehensively updated, but continues to provide simple descriptive explanations of the classic techniques. The purpose remains the same - to teach the chemical, physical and biological principles of fixation, staining and histochemistry and so encourage an intelligent approach to microtechnique.

New procedures that seem likely to become 'standard' methods in research, diagnostic pathology, or the preparation of teaching materials, have been added, including: methods for fixing and processing tissues, guidance on how to make up combinations of staining procedures appropriate to the needs of the moment, in situ hybridization and methods for detecting apoptotic cells.

Histological and histochemical methods for animal tissues still predominate, but the book now includes discussion, with instructions, on the fixing, processing and staining of plants and microorganisms.

This new edition is recommended for: lecturers and students in biological and biomedical disciplines studying histological and histochemical techniques, postgraduate researchers looking for the ideal laboratory companion, medical laboratory technologists preparing for their professional examinations, every histopathology laboratory.

Contents:

1 Introduction to microtechnique 1

2 Fixation 12

3 Decalcification and other treatments for hard tissues 45

4 Processing and mounting 52

5 Dyes 74

6 Histological staining in one or two colours 141

7 Staining blood and other cell suspensions 175

8 Methods for connective tissue 190

9 Methods for nucleic acids 214

10 Organic functional groups and protein histochemistry 240

11 Carbohydrate histochemistry 274

12 Lipids 307

13 Methods for inorganic ions 337

14 Enzyme histochemistry: general considerations 354

15 Hydrolytic enzymes 361

16 Oxidoreductases 386

17 Methods for soluble organic compounds of low molecular weight 425

18 Metal reduction and precipitation methods 440

19 Immunohistochemistry 483

20 Miscellaneous data 520.

Notes:

Includes bibliographical references (pages [534]-580) and index.

ISBN:

9781904842422

1904842429

OCLC:

219969934