Post-translational modifications of proteins : tools for functional proteomics / edited by Christoph Kannicht.

Format:

Book

Contributor:

Kannicht, Christoph.

Series:

Methods in molecular biology ; 446.

Methods in molecular biology ; 446

Language:

English

Subjects (All):

Post-translational modification.

Glycosylation.

Protein Processing, Post-Translational.

Medical Subjects:

Glycosylation.

Protein Processing, Post-Translational.

Physical Description:

xvii, 390 pages : illustrations ; 24 cm.

Edition:

Second edition.

Place of Publication:

Totowa, N.J. : Humana, [2008]

Summary:

The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition, this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting edge, Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition serves as a practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as for scientists in the pharmaceutical industries.

Contents:

1 Disulfide Bond Mapping by Cyanylation-induced Cleavage and Mass Spectrometry / Jiang Wu 1

2 Detection of Post-translational Modifications by Fluorescent Staining of Two-Dimensional Gels / Archana M. Jacob, Chris W. Turck 21

3 Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods / Christoph Weise, Christof Lenz 33

4 Analysis of Tyrosine-O-Sulfation / Jens R. Bundgaard, Jette W. Sen, Anders H. Johnsen, Jens F. Rehfeld 47

5 [alpha]-Amidated Peptides: Approaches for Analysis / Gregory P. Mueller, William J. Driscoll 67

6 [gamma]-Glutamate and [beta]-Hydroxyaspartate in Proteins / Francis J. Castellino, Victoria A. Ploplis, Li Zhang 85

7 Lysine Hydroxylation and Cross-linking of Collagen / Mitsuo Yamauchi, Masashi Shiiba 95

8 Mass Spectrometric Determination of Protein Ubiquitination / Carol E. Parker, Maria R. E. Warren, Viorel Mocanu, Susanna F. Greer, Christoph H. Borchers 109

9 Analysis of Sumoylation / Andrea Pichler 131

10 Detection and Analysis of Protein ISGylation / Tomoharu Takeuchi, Hideyoshi Yokosawa 139

11 Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins / Randy J. Arnold, William Running, James P. Reilly 151

12 Analysis of S-Acylation of Proteins / Michael Veit, Evgeni Ponimaskin, Michael F. G. Schmidt 163

13 Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa / Nahid Azzouz, Peter Gerold, Ralph T. Schwarz 183

14 2-Dimensional Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern / Klemens Loster, Christoph Kannicht 199

15 Carbohydrate Composition Analysis of Glycoproteins by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag / George N. Saddic, Shirish T. Dhume, Kalyan R. Anumula 215

16 Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB) / Rolf Nuck 231

17 Separation of N-Glycans by HPLC / Martin Gohlke, Veronique Blanchard 239

18 Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry-detection of Lewis X Structures / Christoph Kannicht, Detlef Grunow, Lothar Lucka 255

19 Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modification of Proteins / Michael Ahrend, Angela Kaberich, Marie-Therese Fergen, Brigitte Schmitz 267

20 Analysis of O-Glycosylation / Juan J. Calvete, Libia Sanz 281

21 Characterization of Site-specific N-Glycosylation / Katalin F. Medzihradszky 293

22 Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag / Shirish T. Dhume, George N. Saddic, Kalyan R. Anumula 317

23 Comparability and Monitoring Immunogenic N-linked Oligosaccharides from Recombinant Monoclonal Antibodies from Two Different Cell Lines using HPLC with Fluorescence Detection and Mass Spectrometry / Bruce R. Kilgore, Adam W. Lucka, Rekha Patel, Bruce A. Andrien, Jr., Shirish T. Dhume 333

24 Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N-linked Oligosaccharides of Recombinant Monoclonal Antibodies / Adam W. Lucka, Bruce R. Kilgore, Rekha Patel, Bruce A. Andrien, Jr., Shirish T. Dhume 347

25 Web-based Computational Tools for the Prediction and Analysis of Post-translational Modifications of Proteins / Vladimir A. Ivanisenko, Dmitry A. Afonnikov, Nikolay A. Kolchanov 363.

Notes:

Previous ed.: 2002.

Includes bibliographical references and index.

ISBN:

9781588297198

1588297195

9781603270847

1603270841

OCLC:

191891456