2D PAGE : sample preparation and fractionation.

Format:

Book

Contributor:

Posch, Andrew.

Series:

Methods in molecular biology (Clifton, N.J.) ; 424-425.

Methods in molecular biology, 1064-3745 ; v 424-425

Language:

English

Subjects (All):

Proteomics.

Medical Subjects:

Proteomics.

Physical Description:

2 volumes : illustrations ; 25 cm.

Other Title:

Two-dimensional PAGE sample preparation and fractionation

Place of Publication:

Totowa, NJ : Humana Press, [2008]

Summary:

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.

Invaluable for students of Biochemistry, Biomedicine, Biology, and Genomics and essential for practicing scientists, 2D Page: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in-depth methods and protocols to provide a greater understanding of this intricate and complicated field.

Features

Sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics

Provides the most comprehensive collection of sample prep protocols in expression proteomics

Ensures successful results with tricks of the trade and troubleshooting tips

Offers methods that can be applied to a broad range of protein separation techniques

Contents:

Volume 2. Application of fluorescence dye saturation labeling for differential proteome analysis of 1,000 microdissected cells from pancreatic ductal adenocarcinoma precursor lesions / Barbara Sitek ... [et al.]

Albumin and immunoglobulin depletion of human plasma / Rosalind E. Jenkins ... [et al.]

Multi-component immunoaffinity subtraction and reversed-phase chromatography of human serum / James Martosella and Nina Zolotarjova

Immunoaffinity fractionation of plasma proteins by chicken IgY antibodies / Lei Huang and Xiangming Fang

Proteomics of cerebrospinal fluid methods for sample processing / John E. Hale ... [et al.]

Sample preparation of bronchoalveolar lavage fluid / Baptiste Leroy, Paul Falmagne, and Ruddy Wattiez

Preparation of nasal secretions for proteome analysis / Begona Casado, Paolo Iadarola, and Lewis K. Pannell

Preparation of urine samples for proteomic analysis / Rembert Pieper

Isolation of cytoplasmatic proteins from cultured cells for two-dimensional gel electrophoresis / Ying Wang, Jen-Fu Chiu, and Qing-Yu He

Sample preparation of culture medium from Madin-Darby Canine Kidney cells / Daniel Ambort, Daniel Lottaz, and Erwin Sterchi

Sample preparation for mass spectrometry analysis of formalin-fixed paraffin-embedded tissue proteomic analysis of formalin-fixed tissue / Nicolas A. Stewart and Timothy D. Veenstra

Metalloproteomics in the molecular study of cell physiology and disease / Hermann-Josef Thierse, Stefanie Helm, and Patrick Pankert

Protein extraction from green plant tissue / Ragnar Flengsrud

The terminator a device for high-throughput extraction of plant material / B. M. van den Berg

Isolation of mitochondria from plant cell culture / Etienne H. Meyer and A. Harvey Millar

Isolation and preparation of choroplasts from Arabidopsis thaliana plants / Sybille E. Kubis, Kathryn S. Lilley, and Paul Jarvis

Isolation of plant cell wall proteins / Elisabeth Jamet ... [et al.]

Isolation and fractionation of the endoplasmic reticulum from castor bean (Ricinus communis) endosperm for proteomic analyses / William J. Simon, Daniel J. Maltman, and Antoni R. Slabas

Cell wall fractionation for yeast and fungal proteomics / Aida Pitarch, César Nombela, and Concha Gil

Collection of proteins secreted from yeast protoplasts in active cell wall regeneration / Aida Pitarch, César Nombela, and Concha Gil

Sample preparation procedure for cellular fungi / Alois Harder

Isolation and enrichment of secreted proteins from filamentous fungi / Martha L. Medina and Wilson A. Francisco

Isolation and solubilization of cellular membrane proteins from bacteria / Kheir Zuobi-Hasona and L. Jeannine Brady

Isolation and solubilization of gram-positive bacterial cell wall-associated proteins / Jason N. Cole, Steven P. Djordjevic, and Mark J. Walker

Cell fractionation of parasitic protozoa / Wanderley de Souza, José Andrés-Morgado-Diaz, and Narcisa L. Cunha-e-Silva.

Notes:

Includes bilbiographical references and index.

ISBN:

9781588297228

9781603272094

1603272097

OCLC:

225869847