Avian influenza virus / Erica Spackman, editor.

Format:

Book

Contributor:

Spackman, Erica.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 436.

Methods in molecular biology, 1064-3745 ; 436

Language:

English

Subjects (All):

Avian influenza--Research--Methodology.

Avian influenza.

Influenza A virus--genetics.

Research.

Methodology.

Influenza A virus--immunology.

Orthomyxoviridae Infections--virology.

Virology--methods.

Medical Subjects:

Influenza A virus--genetics.

Influenza A virus--immunology.

Orthomyxoviridae Infections--virology.

Virology--methods.

Genre:

Laboratory manuals.

Physical Description:

x, 141 pages : illustrations ; 25 cm.

Place of Publication:

Totowa, NJ : Humana ; London : Springer [distributor], [2008]

Summary:

With the growing global fear of a major pandemic, avian influenza virus research has increased greatly in importance during this young century. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental yet essential virological methods for Al virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. Exciting, cutting-edge new methods focus on studying the virus itself and work with avian hosts, an area greatly lacking in research. Following the format of the highly successful Methods in Molecular Biology series, each chapter provides readily reproducible laboratory protocols offering step-by-step instruction and lists of the necessary equipment for the task. Comprehensive and timely, Avian Influenza Virus equips diagnosticians and researchers with the current tools and information they need to learn more about this high impact disease.

Contents:

A brief introduction to the avian influenza virus / Erica Spackman

Avian influenza virus sample types, collection, and handling / Mary Lea Killian

Avian influenza virus RNA extraction from tissue and swab material / Erica Spackman and David L. Suarez

Type A influenza virus detection and quantitation by real-time RT-PCR / Erica Spackman and David L. Suarez

Detection and identification of the H5 hemagglutinin subtype by real-time RT-PCR

Avian influenza virus isolation and propagation in chicken eggs / Peter R. Woolcock

Hemagglutination assay for the avian influenza virus / Mary Lea Killian

Hemagglutination-inhibition test for avian influenza virus subtype identification and the detection and quantitation of serum antibodies to the avian influenza virus / Janice C. Pedersen

Neuraminidase-inhibition assay for the identification of influenza A virus neuraminidase subtype or neuraminidase antibody specificity / Janice C. Pedersen

Immunohistochemical staining for the detection of the avian influenza virus in tissues / Mary J. Pantin-Jackwood

Wild bird surveillance for the avian influenza virus / Justin D. Brown and David E. Stallknecht

Reverse genetics of the avian influenza virus / Chang-Won Lee and David L. Suarez

Evaluating the cell-mediated immune response of avian species to avian influenza viruses / Darrell R. Kapczynski

Measurement of avian cytokines with real-time RT-PCR following infection with the avian influenza virus / Darrell R. Kapczynski and Michael H. Kogut.

Notes:

Includes bibliographical references and index.

ISBN:

9781588299390

1588299392

9781597452793

1597452793

OCLC:

172984706