Avidin-biotin interactions : methods and applications / edited by Robert J. McMahon.

Format:

Book

Contributor:

McMahon, Robert Joseph, 1958-

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 418.

Methods in molecular biology, 1064-3745 ; 418

Language:

English

Subjects (All):

Biotin.

Avidin.

Affinity chromatography.

Affinity electrophoresis.

Streptavidin.

Protein Binding.

Chemistry, Analytical.

Medical Subjects:

Avidin.

Biotin.

Streptavidin.

Protein Binding.

Chemistry, Analytical.

Genre:

Laboratory manuals.

Physical Description:

xiii, 232 pages : illustrations ; 23 cm.

Place of Publication:

Totowa, NJ : Humana ; [London] : [Springer, distributor], [2008]

Summary:

For researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity associated with popular, low cost, prefabricated kits used by many in the field. In Avidin-Biotin Interactions: Methods and Applications, leaders in avidin-biotin methodology share their knowledge relating to the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. Although avidin-biotin based kits have been produced for quite some time and remain a widely used methodology, many of the chapters in this text describe situations in which the previous methodology failed due to some factor, and the authors developed new and better ways for the avidin-biotin system to be utilized. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab, but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology.

Contents:

1 Preparation of Avidin Conjugates / Rosaria P. Haugland, Mahesh K. Bhalgat 1

2 Coupling of Antibodies With Biotin / Rosaria P. Haugland, Wendy W. You 13

3 Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin / Sara R. Zwart, Brandon J. Lewis 25

4 Use of the Avidin (Imino) Biotin System as a General Approach to Affinity Precipitation / Ruth Freitag, Frank Hilbrig 35

5 Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis / Bao-Shiang Lee, Sangeeth Krishnanchettiar, Syed Salman Lateef, Shalini Gupta 51

6 Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein / Nicolas Humbert, Thomas R. Ward 63

7 Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives / Andreas Ebner, Markus Marek, Karl Kaiser, Gerald Kada, Christoph D. Hahn, Bernd Lackner, Hermann J. Gruber 73

8 Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins / Christoph Rosli, Jascha-N. Rybak, Dario Neri, Giuliano Elia 89

9 High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli / Nicolas Humbert, Peter Schurmann, Andrea Zocchi, Jean-Marc Neuhaus, Thomas R. Ward 101

10 Endogenous Biotin in Rat Brain: Implications for False-Positive Results With Avidin-Biotin and Streptavidin-Biotin Techniques / Bruce E. McKay, Michael L. Molineux, Ray W. Turner 111

11 Pseudo-Immunolabelling With the Avidin-Biotin-Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina / Willem Kamphuis, Jan Klooster 129

12 Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones / Gabriela Camporeale, Yap Ching Chew, Alice Kueh, Gautam Sarath, Janos Zempleni 139

13 Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay / Kirsten Liebert, Albert Jeltsch 149

14 Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin / Elisabeth D. Coene, Michael K. Shaw, David J. Vaux 157

15 Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure / Karel Smetana, Jr., Sabine Andre 171

16 A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin / Krista McCutcheon 187

17 Biotin-Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications / Donald M. Mock, Anna Bogusiewicz 209.

Notes:

Includes bibliographical references and index.

ISBN:

9781588295835

1588295834

9781597455794

1597455792

OCLC:

213105680