Genomics protocols / edited by Mike Starkey, Ramnath Elaswarapu.

Format:

Book

Contributor:

Starkey, Michael P.

Elaswarapu, Ramnath.

Series:

Methods in molecular biology (Clifton, N.J.) ; 439.

Methods in molecular biology ; 439

Language:

English

Subjects (All):

Molecular genetics--Laboratory manuals.

Molecular genetics.

Gene mapping--Laboratory manuals.

Gene mapping.

Genomics--methods.

Genetic Techniques.

Chromosome Mapping--methods.

Sequence Analysis, DNA--methods.

Molecular Biology--methods.

Medical Subjects:

Genomics--methods.

Genetic Techniques.

Chromosome Mapping--methods.

Sequence Analysis, DNA--methods.

Molecular Biology--methods.

Genre:

Laboratory manuals.

Physical Description:

xv, 425 pages : illustrations ; 25 cm.

Edition:

Second edition.

Place of Publication:

Totowa, NJ : Humana Press, [2008]

Summary:

Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, Second Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, Second Edition is the perfect update for researchers working in this integral, growing field.

Contents:

Whole genome amplification with Phi29 DNA polymerase to enable genetic or genomic analysis of samples of low DNA yield / Kaisa Silander and Janna Saarela

Scanning for DNA variants by denaturant capillary electrophoresis / Per O. Ekstrøm

Identification of SNPs, or mutations in sequence chromatograms / Nicole Draper

BeadArray-based genotyping / Helen Butler and Jiannis Ragoussis

Microsatellite-based candidate gene linkage analysis studies / Cathryn Mellersh

Full complexity genomic hybridization on 60-mer oligonucleotide microarrays for array comparative genomic hybridization (aCGH) / Alexei Protopopov, Bin Feng, and Lynda Chin

Detection of copy number changes at multiple loci in DNA prepared from formalin-fixed, paraffin-embedded tissue by multiplex ligation -dependent probe amplification / Minoru Takata

Application of microarrays for DNA methylation profiling / Axel Schumacher, Andreas Weinhäusl, and Arturas Petronis

Genomewide identification of protein binding locations using chromatin immunoprecipitation coupled with microarray / Byung-Kwan Cho, Eric M. Knight, and Bernhard Ø. Palsson

Transcriptional profiling of small samples in the central nervous system / Stephen D. Ginsberg

Quantitative expression profiling of RNA from formalin-fixed, paraffin-embedded tissues using randomly assembled bead arrays / Marina Bibikova ... [et al.]

Expression profiling of microRNAs in cancer cells : technical considerations / Mouldy Sioud and Lina Cekaite

Identification of disease biomarkers by profiling of serum proteins using SELDI-TOF mass spectrometry / Sigrun Langbein

The applicability of a cluster of differentiation monoclonal antibody microarray to the diagnosis of human disease / Peter Ellmark ... [et al.]

Protein profiling based on two-dimensional difference gel electrophoresis / Gert Van den Bergh and Lutgarde Arckens

Quantitative protein profiling by mass spectrometry using isotope-coded affinity tags / Arsalan S. Haqqani, John F. Kelly, and Danica B. Stanimirovic

Quantitative protein profiling by mass spectrometry using label-free proteomics / Arsalan S. Haqqani, John F. Kelly, and Danica B. Stanimirovic

Using 2D-LC-MS/MS to identify Francisella tularensis peptides in extracts from an infected mouse macrophage cell line / John F. Kelly and Wen Ding

Baculovirus expression vector system : an emerging host for high-throughput eukaryotic protein expression / Binesh Shrestha, Carol Smee, and Opher Gileadi

Coimmunoprecipitation and proteomic analyses / S. Fabio Falsone, Bernd Gesslbauer, and Andreas J. Kungl

Tandem affinity purification combined with mass spectrometry to identify components of protein complexes / Peter Kaiser ... [et al.]

Mammalian two-hybrid assay for detecting protein-protein interactioins in vivo / Runtao He and Xuguang Li

Detection of protein-protein interactions in live cells and animals with split firefly luciferase protein fragment complementation / Victor Villalobos, Snehal Naik, and David Piwnica-Worms

Subcellular localization of intracellular human proteins by construction of tagged fusion proteins and transient expression in COS-7 cells / John E. Collins

GeneFAS : a tool for the prediction of gene function using multiple sources of data / Trupti Joshi ... [et al.]

Comparative genomics-based prediction of protein function / Toni Gabaldón

Design, manufacture, and assay of the efficacy of siRNAs for gene silencing / Louise A. Dawson and Badar A. Usmani.

Notes:

Includes bibliographical references and index.

ISBN:

9781588298713

158829871X

OCLC:

178312599