Organelle proteomics / edited by Delphine Pflieger and Jean Rossier.

Format:

• Book

Contributor:

• Pflieger, Delphine.
• Rossier, Jean.

Series:

• Methods in molecular biology (Clifton, N.J.) ; v. 432.
• Methods in molecular biology, 1064-3745 ; 432

Language:

English

Subjects (All):

• Cell organelles.
• Proteomics.
• Organelles.
• Proteomics--methods.

Medical Subjects:

• Organelles.
• Proteomics--methods.

Genre:

• Laboratory manuals.

Physical Description:

xvii, 421 pages : illustrations ; 25 cm.

Place of Publication:

Totowa, NJ : Humana ; London : Springer [distributor], [2008]

Summary:

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.

Contents:

• 1 Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry / Edwin P. Romijn, John R. Yates III 1
• Part I Analysis of Organelles, Vesicles, and Protein Machineries
• 2 Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments / Daniel Salvi, Norbert Rolland, Jacques Joyard, Myriam Ferro 19
• 3 Proteomic Analysis of the Stacked Golgi Complex / Mark S. Lowenthal, Kathryn E. Howell, Christine C. Wu 37
• 4 Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device / Hans Zischka, Norbert Kinkl, Ralf J. Braun, Marius Ueffing 51
• 5 Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria: Activity Measurement and Subunit Composition Analysis / Claire Lemaire, Genevieve Dujardin 65
• 6 Mitochondrial Proteomics: Analysis of a Whole Mitochondrial Extract with Two-Dimensional Electrophoresis / Thierry Rabilloud 83
• 7 Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane / Sandrine Da Cruz, Jean-Claude Martinou 101
• 8 Subcellular Fractionation and Proteomics of Nuclear Envelopes / Laurence Florens, Nadia Korfali, Eric C. Schirmer 117
• 9 Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction / Tsuneyoshi Horigome, Kazuhiro Furukawa, Kohei Ishii 139
• 10 Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis / Kiichi Fukui, Hideaki Takata, Susumu Uchiyama 149
• 11 Purification and Proteomic Analysis of Plant Plasma Membranes / Erik Alexandersson, Niklas Gustavsson, Katja Bernfur, Adine Karlsson, Per Kjellbom, Christer Larsson 161
• 12 Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues / Jacek R. Wisniewski 175
• 13 Proteomic Characterization of Membrane Protein Topology / Adele R. Blackler, Christine C. Wu 185
• 14 Free Flow Isoelectric Focusing: A Method for the Separation of Both Hydrophilic and Hydrophobic Proteins of Rat Liver Peroxisomes / Markus Islinger, Gerhard Weber 199
• 15 Use of Gas-Phase Fractionation to Increase Protein Identifications: Application to the Peroxisome / Jacob Kennedy, Eugene C. Yi 217
• 16 Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins / Huiwen Zhang, Xiaolian Fan, Rick Bagshaw, Don J. Mahuran, John W. Callahan 229
• 17 Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification / Sylvie Kieffer-Jaquinod, Agnes Chapel, Jerome Garin, Agnes Journet 243
• 18 Purification and Proteomic Analysis of Synaptic Vesicles / Holly D. Cox, Charles M. Thompson 259
• 19 Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes / Xuequn Chen, Philip C. Andrews 275
• 20 Isolation and Proteomic Analysis of Chlamydomonas Centrioles / Lani C. Keller, Wallace F. Marshall 289
• 21 Purification and Proteomic Analysis of 20S Proteasomes from Human Cells / Marie-Pierre Bousquet-Dubouch, Sandrine Uttenweiler-Joseph, Manuelle Ducoux-Petit, Mariette Matondo, Bernard Monsarrat, Odile Burlet-Schiltz 301
• 22 Characterization of E. coli Ribosomal Particles: Combined Analysis of Whole Proteins by Mass Spectrometry and of Proteolytic Digests by Liquid Chromatography-Tandem Mass Spectrometry / Isabelle Iost, Julie Charollais, Joelle Vinh, Delphine Pflieger 321
• Part II Proteins Identified in the Organelle Sample: True Components or Contaminants?
• 23 Assessment of Organelle Purity Using Antibodies and Specific Assays: The Example of the Chloroplast Envelope / Daniel Salvi, Norbert Rolland, Jacques Joyard, Myriam Ferro 345
• 24 Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions: An Example in Peroxisomes / Marcello Marelli, Alexey I. Nesvizhskii, John D. Aitchison 357
• 25 Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics / Kathryn S. Lilley, Tom P. J. Dunkley 373
• 26 Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins / Wei Yan, Daehee Hwang, Ruedi Aebersold 389
• 27 Use of Transcriptomic Data to Support Organelle Proteomic Analysis / Wallace F. Marshall 403.

Notes:

Includes bibliographical references and index.

ISBN:

• 9781588297792
• 1588297799

OCLC:

178312727