Microbial gene essentiality : protocols and bioinformatics / edited by Andrei L. Osterman and Svetlana Y. Gerdes.

Format:

Book

Contributor:

Osterman, Andrei L.

Gerdes, Svetlana Y.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 416.

Methods in molecular biology ; 416

Language:

English

Subjects (All):

Microbial genetics.

Genetics, Microbial.

Medical Subjects:

Genetics, Microbial.

Physical Description:

xiv, 486 pages : illustrations ; 27 cm.

Place of Publication:

Totowa, N.J. : Humana Press, 2008.

Contents:

Overview of whole-genome essentiality analysis / Karen Joy Shaw

Pt. I. Experimental protocols. IA. Populational genome-wide essentiality screens. Transposon-based strategies for the identification of essential bacterial genes / William S. Reznikoff and Kelly M. Winterberg

Identification and analysis of essential genes in Haemophilus influenzae / Sandy M.S. Wong and Brian J. Akerley

Transposon site hybridization in Mycobacterium tuberculosis / Jeffrey P. Murry ... [et al.]

Essential genes in the infection model of Pseudomonas aeruginosa PCR-based signature-tagged mutagenesis / François Sanschagrin, Irena Kukavica-Ibrulj, and Roger C. Levesque

Whole-genome detection of conditionally essential and dispensable genes in Escherichia coli via genetic footprinting / Michael D. Scholle and Svetlana Y. Gerdes

Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis / Taeok Bae ... [et al.]

Multipurpose transposon insertion libraries for large-scale analysis of gene function in yeast / Anuj Kumar

IB. Systematic collections of knockout mutants. How to make a defined near-saturation mutant library. Case 1: Pseudomonas aeruginosa PAO1 / Michael A. Jacobs

Comparing insertion libraries in two Pseudomonas aeruginosa strains to assess gene essentiality / Nicole T. Liberati ... [et al.]

The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12 / Tomoya Baba and Hirotada Mori

The applications of systematic in-frame, single-gene knockout mutant collection of Escherichia coli K-12 / Tomoya Baba ... [et al.]

A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12 / Takeyoshi Miki, Yoshihiro Yamamoto, and Hideo Matsuda

High-throughput creation of a whole-genome collection of yeast knockout strains / Angela M. Chu and Ronald W. Davis

Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray / Pamela B. Meluh ... [et al.]

IC. Genome minimization. Scarless engineering of the Escherichia coli genome / Tamás Fehér ... [et al.]

Minimization of the Escherichia coli genome using the Tn5-targeted Cre/loxP excision system / Byung Jo Yu and Sun Chang Kim

Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome / Jun-ichi Kato and Masayuki Hashimoto

ID. Conditional knockouts. Identification of essential genes in staphylococcus aureus by construction and screening of conditional mutant library / Dezhong Yin and Yinduo Ji

Techniques for the isolation and use of conditionally expressed antisense RNA to achieve essential gene knockdowns in Staphylococcus aureus / Allyn Forsyth and Liangsu Wang

Introduction of conditional lethal amber mutations in Escherichia coli / Christopher D. Herring

Pt. II. Bioinformatics. IIA. Statistics. Statistical methods for building random transposon mutagenesis libraries / Oliver Will

Statistical evaluation of genetic footprinting data / Gábor Balázsi

Modeling competitive outgrowth of mutant populations: why do essentiality screens yield divergent results? / Alexander I. Grenov and Svetlana Y. Gerdes

Statistical analysis of fitness data determined by TAG hybridization on microarrays / Brian D. Peyser, Rafael Irizarry, and Forrest A. Spencer

IIB. Data integration and modeling. Profiling of Escherichia coli chromosome database / Yukiko Yamazaki, Hironori Niki, and Jun-ichi Kato

Gene essentiality analysis based on DEG, a database of essential genes / Chun-Ting Zhang and Ren Zhang

Detection of essential genes in Streptococcus pneumoniae using bioinformatics and allelic replacement mutagenesis / Jae-Hoon Song and Kwan Soo Ko

Design and application of genome-scale reconstructed metabolic models / Isabel Rocha, Jochen Förster, and Jens Nielsen

Predicting gene essentiality using genome-scale in silico models / Andrew R. Joyce and Bernhard Ø. Palsson

Comparative approach to analysis of gene essentiality / Andrei L. Osterman and Svetlana Y. Gerdes.

Notes:

Includes bibliographical references and index.

ISBN:

9781588293787

1588293785

OCLC:

209342501