Neural stem cells : methods and protocols.

Format:

Book

Contributor:

Weiner, Leslie P.

Series:

Methods in molecular biology ; 438.

Methods in molecular biology ; 438

Language:

English

Subjects (All):

Neurons--Laboratory manuals.

Neurons.

Stem cells--Laboratory manuals.

Stem cells.

Stem Cells.

Medical Subjects:

Neurons.

Stem Cells.

Genre:

Laboratory manuals.

Physical Description:

xv, 406 pages : illustrations ; 24 cm.

Edition:

Second edition / edited by Leslie P. Weiner.

Place of Publication:

Totowa, N.J. : Humana, 2008.

Summary:

Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells: Methods and Protocols, Second Edition, revises and expands on the successful first edition in order to provide the most current, cutting-edge methods for scientists working to answer these questions. Using these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means for understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new edition provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Neural Stem Cells: Methods and Protocols, Second Edition, is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

Contents:

1 Definitions and Criteria for Stem Cells / Leslie P. Weiner 3

2 Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard / Jaime Imitola, Samia J. Khoury 9

Part II Isolation and Culture of NSCs

3 Neural Differentiation of Human Embryonic Stem Cells / Mirella Dottori, Martin F. Pera 19

4 Generating Neurons from Stem Cells / Andreas Androutsellis-Theotokis, Sachiko Murase, Justin D. Boyd, Deric M. Park, Daniel J. Hoeppner, Rea Ravin, Ronald D. G. McKay 31

5 Isolation of Neural Stem and Precursor Cells from Rodent Tissue / Yuanyuan Wu, Ying Liu, Jonathan D. Chesnut, Mahendra S. Rao 39

6 Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization / Lamya S. Shihabuddin 55

7 Adult Neural Stem Cells / Rossella Galli, Angela Gritti, Angelo L. Vescovi 67

8 Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb / Susan C. Barnett, A. Jane Roskams 85

9 Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium / Miranda Richter, Kathryn Westendorf, A. Jane Roskams 95

10 Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property / Seung U. Kim, Atsushi Nagai, Eiji Nakagawa, Hyun B. Choi, Jung H. Bang, Hong J. Lee, Myung A. Lee, Yong B. Lee, In H. Park 103

11 Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood / Shijie Song, Juan Sanchez-Ramos 123

12 Production of Neurospheres from CNS Tissue / Gregory P. Marshall II, Heather H. Ross, Oleg Suslov, Tong Zheng, Dennis A. Steindler, Eric D. Laywell 135

13 Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells / Shigeo Saito, Ken Sawai, Yoshinobu Murayama, Keiichi Fukuda, Kazunari Yokoyama 151

Part III In Vitro Studies: A. Cellular Techniques

14 Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures / Angela Gritti, Rossella Galli, Angelo L. Vescovi 173

15 Telomere Neurobiology / Mark P. Mattson, Peisu Zhang, Aiwu Cheng 185

16 Chromosomal Mosaicism in Neural Stem Cells / Suzanne E. Peterson, Jurjen W. Westra, Christine M. Paczkowski, Jerold Chun 197

17 In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype / Marcel M. Daadi 205

18 In Vitro Migration Assays of Neural Stem Cells / Pascale Durbec, Isabelle Franceschini, Francoise Lazarini, Monique Dubois-Dalcq 213

19 Measuring Apoptosis in Neural Stem Cells / Brett T. Lund, Eve E. Kelland 227

20 Markers of Adult Neural Stem Cells / Eric Wexler 243

Part III In Vitro Studies: B. Molecular Techniques

21 Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR / Nicole Slawny, Crystal Pacut, Theresa E. Gratsch 271

22 Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells / Yan Chen, Jiang F. Zhong 293

23 Electroporation Strategies for Genetic Manipulation and Cell Labeling / Terence J. Van Raay, Rhonda T. Lassiter, Michael R. Stark 305

24 Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches / Luciano Conti, Elena Cattaneo 319

Part IV Studies for Transplantation

25 Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo / Sanjay S. Magavi, Jeffrey D. Macklis 335

26 Immunocytochemical Analysis of Neuronal Differentiation / Sanjay S. Magavi, Jeffrey D. Macklis 345

27 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold / Lisa A. Catapano, Sanjay S. Magavi, Jeffrey D. Macklis 353

28 Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS / Qi-lin Cao, Stephen M. Onifer, Scott R. Whittemore 361

29 Optimizing Stem Cell Grafting into the CNS / Scott R. Whittemore, Yi Ping Zhang, Christopher B. Shields, David S. K. Magnuson 375

30 Routes of Stem Cell Administration in the Adult Rodent / Alison E. Willing, Svitlana Garbuzova-Davis, Paul R. Sanberg, Samuel Saporta 383.

Notes:

Previous ed.: 2002.

Includes bibliographical references and index.

ISBN:

9781588298461

1588298469

OCLC:

187294476