Bioactive natural products : detection, isolation, and structural determination / edited by Steven M. Colegate, Russell J. Molyneux.

Format:

Book

Contributor:

Colegate, Steven M.

Molyneux, Russell J.

Rosengarten Family Fund.

Language:

English

Subjects (All):

Natural products.

Bioactive compounds.

Physical Description:

xiii, 605 pages : illustrations ; 27 cm

Edition:

Second edition.

Place of Publication:

Boca Raton : CRC Press, [2008]

Summary:

Bioactive natural products are proving to be a rich source of novel therapeutics to both protect against and combat diseases, as well as serve as lead compounds in crop protection. Following the successful format of the first edition, Bioactive Natural Products: Detection, Isolation, and Structural Determination, Second Edition brings together collective research of many new contributors and emphasizes the rationale behind the successful detection, isolation and structure determination of specific compounds.

The text encompasses a diverse range of techniques that can be applied to terrestrial and aquatic sources. It also allows you to understand how source material can be selected to enhance your opportunity for novel bioactive natural products. New to the Second Edition- Advances in the application of NMR spectroscopy and mass spectrometry, bioactive chemical detection and extraction, and novel bioassay development, New case studies and illustrations that demonstrate the practical applications of specific techniques, A group of new contributors presenting research from their own laboratories that emphasize both the philosophy and rationale behind detection, isolation, and structural determination.

Following an overview of natural product chemistry techniques and approaches, many new chapters discuss selection of source material, quantitative NMR, high speed counter-current chromatography, dereplication of extracts, and methods to determine the stereochemistry of bioactive natural products. Examinations of new technologies including LC-NMR, biosensors, and biofingerprinting accompany discussions on the search for specific activities in anticancer and antimalarial applications, seed germination stimulation, and mammalian toxicity.

With participation from active researchers, this definitive work supplies a vital extension and an enduring contribution to the science and art of bioactive natural product detection, isolation, and structural determination.

Contents:

Chapter 1 An Introduction and Overview / Steven M. Colegate, Russell J. Molyneux 1

Chapter 2 Detection and Isolation of Bioactive Natural Products / Emilio L. Ghisalberti 11

Chapter 3 Nuclear Magnetic Resonance Spectroscopy: Strategies for Structural Determination / Lindsay T. Byrne 77

Chapter 4 Quantitative NMR of Bioactive Natural Products / Guido F. Pauli, Birgit U. Jaki, David C. Lankin, John A. Walter, Ian W. Burton 113

Chapter 5 Development and Application of LC-NMR Techniques to the Identification of Bioactive Natural Products / Jean-Luc Wolfender, Emerson F. Queiroz, Kurt Hostettmann 143

Chapter 6 Determination of the Absolute Configuration of Bioactive Natural Products Using Exciton Chirality Circular Dichroism / Hans-Ulrich Humpf 191

Chapter 7 Separation of Enantiomeric Mixtures of Alkaloids and Their Biological Evaluation / Stephen T. Lee, Russell J. Molyneux, Kip E. Panter 209

Chapter 8 Dereplication and Discovery of Natural Products by UV Spectroscopy / Thomas O. Larsen, Michael A.E. Hansen 221

Chapter 9 Liquid Chromatography-Mass Spectrometry in Natural Product Research / Ka-Wing Cheng, Feng Chen, Mingfu Wang 245

Chapter 10 Application of High-Speed Countercurrent Chromatography to the Isolation of Bioactive Natural Products / Huabin Li, Feng Chen 267

Chapter 11 Biosensing Approach in Natural Product Research / Maria Minunni, Anna R. Bilia 299

Chapter 12 Anticancer Drug Discovery and Development from Natural Products / Gordon M. Cragg, David J. Newman 323

Chapter 13 Sourcing Natural Products from Endophytic Microbes / Gary Strobel, Uvidelio Castillo 371

Chapter 14 Isolation of Bioactive Natural Products from Myxobacteria / Makoto Ojika 391

Chapter 15 Naturally Occurring Glycosidase Inhibitors / Robert J. Nash 407

Chapter 16 Bioassay-Directed Isolation and Identification of Antiaflatoxigenic Constituents of Walnuts / Russell J. Molyneux, Noreen Mahoney, Jong H. Kim, Bruce C. Campbell 421

Chapter 17 Bioactive Peptides in Hen Eggs / Yoshinori Mine, Icy D'Silva 439

Chapter 18 Biological Fingerprinting Analysis: Strategy for Screening Bioactive Compounds in Traditional Chinese Medicines / Xingye Su, Hanfa Zou, Xiaoyuan Lei, Liang Kong, Lianghai Hu 459

Chapter 19 Antimalarial Compounds from Traditionally Used Medicinal Plants / Lengo Mambu, Philippe Grellier 491

Chapter 20 Germination Stimulant in Smoke: Isolation and Identification / Gavin R. Flematti, Emilio L. Ghisalberti, Kingsley W. Dixon, Robert D. Trengove 531

Chapter 21 Plant-Associated Toxins: Bioactivity-Guided Isolation, ELISA, and LC-MS Detection / Steven M. Colegate 555.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Rosengarten Family Fund.

ISBN:

9780849372582

0849372585

OCLC:

85851543

Online:

Publisher description