Neuropeptide techniques / edited by Illana Gozes.

Format:

Book

Contributor:

Gozes, Illana.

Series:

Neuromethods ; 39.

Neuromethods ; 39

Language:

English

Subjects (All):

Neuropeptides.

Neuropeptides--physiology.

Neuropeptides--chemical synthesis.

Medical Subjects:

Neuropeptides--physiology.

Neuropeptides--chemical synthesis.

Physical Description:

xii, 160 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2008]

Summary:

Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function. With a selected description of techniques in neuropeptide research, Neuropeptide Techniques covers aspects of design, synthesis and biological evaluation of peptides and peptide analogues. Written by leaders in the field, this book describes primary cell cultures and cell lines, as well as techniques in neuropeptide processing, trafficking and secretion. In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice are described. Neuropeptide Techniques provides the reader with broad perspectives and breadth of knowledge on current topics related to neuropeptide research: A selected description of techniques in neuropeptide research written by leaders in the field, Covers design synthesis and biological evaluation of peptides and peptide analogues, Emphasis on advanced technologies of transfection transgenic delivery and bioinformatics, Primary cells and cell lines techniques in neuropeptide processing, trafficking, and secretion are discussed, Examines in vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice.

Contents:

1 The Design, Synthesis, and Biological Evaluation of VIP and VIP Analogs / David Dangoor, Sara Rubinraut, Mati Fridkin, Illana Gozes 1

2 Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example / Illana Gozes, Miri Holtser-Cochav, Karin Vered 11

3 Primary Cell Cultures and Cell Lines / Inna Divinski, Inbar Pilzer, Illana Gozes 21

4 Transfection of DNA into Cells / Inbar Pilzer, Inna Divinski, Illana Gozes 27

5 Transgenic Delivery and Detection of GFP in Neuropeptide Neurons / J.L. Holter, J.S. Davies, P.-S. Man, T. Wells, D.A. Carter 31

6 Integrative Technologies for Neuropeptide Characterization / Sergey E. Ilyin, Carlos R. Plata-Salaman 45

7 Techniques in Neuropeptide Processing, Trafficking, and Secretion / Niamh X. Cawley, Tulin Yanik, Irina Arnaoutova, Hong Lou, Nimesh Patel, Y. Peng Loh 67

8 In vivo Techniques Quantifying Blood-Brain Barrier Permeability to Small Proteins in Mice / Weihong Pan, Abba J. Kastin 97

9 Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs / Terry W. Moody, Michael Schumann, Robert T. Jensen 115

10 Developmental Milestones in the Newborn Mouse / Joanna M. Hill, Maria A. Lim, Madeleine M. Stone 131.

Notes:

Includes bibliographical references and index.

ISBN:

1588294684

9781588294685

OCLC:

149146464