Real-time PCR in microbiology : from diagnosis to characterization / [edited by] Ian M. Mackay.

Format:

Book

Contributor:

Mackay, Ian M. (Ian Maxwell)

Language:

English

Subjects (All):

Medical microbiology.

Polymerase chain reaction.

Molecular diagnosis.

Polymerase Chain Reaction.

Medical Subjects:

Polymerase Chain Reaction.

Physical Description:

x, 454 pages : illustrations ; 24 cm

Place of Publication:

Norfolk, UK : Caister Academic, [2007]

Summary:

Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This invaluable book describes and explains some of the more complex aspects of real-time PCR presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardization, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion, providing an insightful, topical, and interesting discourse with contributions from over 30 authorities and experts on real-time PCR.

The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. A recommended book for all microbiology laboratories.

Contents:

1 Real-time PCR: History and Fluorogenic Chemistries / Ian M. Mackay, John F. Mackay, Michael D. Nissen, Theo P. Sloots 1

2 Oligonucleotide Design for In-house Real-time PCR Applications in Microbiology / Andreas Nitsche 41

3 QPCR: Target Preparation / Tania Nolan, Reinhold Mueller, Stephen Bustin 71

4 Standards and Controls: Concepts for Preparation and Use in Real-time PCR Applications / Amy Muska, Edith Peck, Stuart Palmer 101

5 Quantification of Microorganisms: not Human, not Simple, not Quick / Ian M. Mackay, Stephen A. Bustin, Jose Manuel Andrade, Mikael Kubista, Theo P. Sloots 133

6 Multiplex rtPCR in Microbiology / Nick M. Cirino, Norma P. Tavakoli, Susan Madison-Antenucci, Christina Egan 183

7 The Role of Real-time PCR in Routine Microbial Diagnostics / Eric C.J. Claas, Willem. J.G. Melchers, Adriaan J.C. van den Brule 231

8 Challenges Facing Real-time PCR Characterization of Acute Respiratory Tract Infections / Ian M. Mackay, Katherine E. Arden, Michael D. Nissen, Theo P. Sloots 269

9 Rapid Detection of Bioterror Agents / Andreas Nitsche 319

10 Experts' Roundtable: Real-Time PCR and Microbiology / M.G.H.M. Beld, C. Birch, P.A. Cane, W. Carman, E.C.J. Claas, J.P. Clewley, E. Domingo, J. Druce, C. Escarmis, R.A.M. Fouchier, V. Foulongne, M.G. Ison, L.C. Jennings, B. Kaltenboeck, I.D. Kay, M. Kubista, O. Landt, I.M. Mackay, J. Mackay, H.G.M. Niesters, M.D. Nissen, S. Palladino, N.G. Papadopoulos, A. Petrich, M.W. Pfaffl, W. Rawlinson, U. Reischl, N.A. Saunders, C. Savolainen-Kopra, O. Schildgen, G.M. Scott, M. Segondy, R. Seibl, T.P. Sloots, Y.-W. Tang, R. Tellier, P.C.Y. Woo 357.

Notes:

Includes bibliographical references and index.

ISBN:

1904455182

9781904455189

OCLC:

153576580