Innate immunity / edited by Jonathan Ewbank, Eric Vivier.

Format:

Book

Contributor:

Ewbank, Jonathan.

Vivier, E.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 415.

Methods in molecular biology ; 415

Language:

English

Subjects (All):

Natural immunity.

Immunology, Comparative.

Plant immunology.

Immunity, Innate--physiology.

Medical Subjects:

Immunity, Innate--physiology.

Physical Description:

xiv, 458 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2008]

Summary:

Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens. Innate Immunity therefore contains chapters dealing with the isolation and functional characterization of cells involved in innate immunity in mouse and man, including mast cells and eosinophils, and with several chapters focusing on natural killer cells. Other chapters describe methods in statistics, in vivo imaging, genome engineering, mutagenesis and culture that are particularly adapted to the study of innate immunity in these hosts. These are complemented with a series of chapters dealing with alternative models: plants, worms, mosquitos, flies, and fish. These approaches and models are being used to dissect the complex interplay between hosts and pathogens, and contribute to developing strategies to help fight infection. With chapters written by experts on the cutting-edge of this technology, Innate Immunity is an essential reference for immunologists, histologists, geneticists, and molecular biologists.

Features: Contains chapters dealing with the isolation and functional characterization of cells involved in innate immunity in mouse and man, including natural killer cells, mast cells, and eosinophils, Several chapters focus on alternative and emerging models, Chapters written by leaders in the field, Follows the successful Methods in Molecular Biology[Trademark] series format offering step-by-step laboratory instructions.

Contents:

1 ENU Mutagenesis in Mice / Philippe Georgel, Xin Du, Kasper Hoebe, Bruce Beutler 1

2 Innate Immunity Genes as Candidate Genes: Searching for Relevant Natural Polymorphisms in Databases and Assessing Family-Based Association of Polymorphisms with Human Diseases / Pascal Rihet 17

3 KIR Locus Polymorphisms: Genotyping and Disease Association Analysis / Maureen P. Martin, Mary Carrington 49

4 Experimental Model for the Study of the Human Immune System: Production and Monitoring of "Human Immune System" Rag[Characters not reproducible]Mice / Nicolas Legrand, Kees Weijer, Hergen Spits 65

5 Bacterial Artificial Chromosome Transgenesis Through Pronuclear Injection of Fertilized Mouse Oocytes / Kristina Vintersten, Giuseppe Testa, Ronald Naumann, Konstantinos, Anastassiadis, A. Francis Stewart 83

6 Bioluminescence Imaging to Evaluate Infections and Host Response In Vivo / Pamela Reilly Contag 101

7 Intravital Two-Photon Imaging of Natural Killer Cells and Dendritic Cells in Lymph Nodes / Susanna Celli, Beatrice Breart, Philippe Bousso 119

8 Dissection of the Antiviral NK Cell Response by MCMV Mutants / Stipan Jonjic, Astrid Krmpotic, Jurica Arapovic, Ulrich H. Koszinowski 127

9 Analyzing Antibody-Fc-Receptor Interactions / Falk Nimmerjahn, Jeffrey V. Ravetch 151

10 The Isolation and Identification of Murine Dendritic Cell Populations from Lymphoid Tissues and Their Production in Culture / David Vremec, Ken Shortman 163

11 Analysis of Individual Natural Killer Cell Responses / Wayne M. Yokoyama, Sungjin Kim 179

12 Isolation and Analysis of Human Natural Killer Cell Subsets / Guido Ferlazzo 197

13 Innate Immune Function of Eosinophils: From Antiparasite to Antitumor Cells / Fanny Legrand, Gaetane Woerly, Virginie Driss, Monique Capron 215

14 Ex Vivo and In Vitro Primary Mast Cells / Michel Arock, Alexandra Le Nours, Odile Malbec, Marc Daeron 241

15 Murine Macrophages: A Technical Approach / Luisa Martinez-Pomares, Siamon Gordon 255

16 Clinical Analysis of Dendritic Cell Subsets: The Dendritogram / Anne Hosmalin, Miriam Lichtner, Stephanie Louis 273

17 Clinical Analysis of Human Natural Killer Cells / Pascale Andre, Nicolas Anfossi 291

18 Lentiviral Transduction of Immune Cells / Louise Swainson, Cedric Mongellaz, Oumeya Adjali, Rita Vicente, Naomi Taylor 301

19 Axenic Mice Model / Antoine Giraud 321

20 In Vivo Analysis of Zebrafish Innate Immunity / Jean-Pierre Levraud, Emma Colucci-Guyon, Michael J. Redd, Georges Lutfalla, Philippe Herbomel 337

21 Reverse Genetics Analysis of Antiparasitic Responses in the Malaria Vector, Anopheles gambiae / Stephanie A. Blandin, Elena A. Levashina 365

22 Drosophila Immunity: Methods for Monitoring the Activity of Toll and Imd Signaling Pathways / Yves Romeo, Bruno Lemaitre 379

23 Investigating the Involvement of Host Factors Involved in Intracellular Pathogen Infection by RNAi in Drosophila Cells / Herve Agaisse 395

24 Models of Caenorhabditis elegans Infection by Bacterial and Fungal Pathogens / Jennifer R. Powell, Frederick M. Ausubel 403

25 Genetic Analysis of Caenorhabditis elegans Innate Immunity / Michael Shapira, Man-Wah Tan 429

26 Measuring Cell-Wall-Based Defenses and Their Effect on Bacterial Growth in Arabidopsis / Min Gab Kim, David Mackey 443.

Notes:

Includes bibliographical references and index.

ISBN:

9781588297464

OCLC:

171552198