Chromosomal mutagenesis / edited by Gregory D. Davis, Kevin J. Kayser.

Format:

Book

Contributor:

Davis, Gregory D.

Kayser, Kevin J.

Series:

Methods in molecular biology (Clifton, N.J.) ; 435.

Methods in molecular biology ; 435

Language:

English

Subjects (All):

Mutagenesis.

Genetic engineering.

Chromosomes.

Genetic Engineering.

Mutagenesis--Laboratory manuals.

Chromosomes--Laboratory manuals.

Genetic engineering--Laboratory manuals.

Medical Subjects:

Mutagenesis.

Chromosomes.

Genetic Engineering.

Genre:

Laboratory manuals.

Physical Description:

xvi, 235 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2008]

Summary:

Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology[Trademark] format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types. Features: Comprehensive and cutting-edge, Highlights techniques involving a wider variety of cell types and organisms, Contains state-of-the-art methods in step-by-step laboratory format, Aims to expand the genetic toolbox beyond model organisms.

Contents:

Biallelic gene knockouts in Chinese hamster ovary cells / Naoko Yamane-Ohnuki, Kazuya Yamano, and Mitsuo Satoh

Highly proficient gene targeting by homologous recombination in the human pre-B cell line Nalm-6 / Noritaka Adachi, Aya Kurosawa, and Hideki Koyama

Robust cell line development using meganucleases / Jean-Pierre Cabaniols and Frédéric Pâques

Design and testing of zinc finger nucleases for use in mammalian cells / Matthew Porteus

Gene targeting in Drosophila and Caenorhabditis elegans with zinc-finger nucleases / Dana Carroll ... [et al.]

Orpheus recombination : a comprehensive bacteriophage system for murine targeting vector construction by transplacement / Knut Woltjen ... [et al.]

Transposon -mediated mutagenesis in somatic cells : identification of transposon-genomic DNA junctions / David A. Largaespada and Lara S. Collier

Insertional mutagenesis of the mouse germline with sleeping beauty transposition / Junji Takeda, Zsuzsanna Izsvák, and Zoltán Ivics

Conditional gene trapping using the FLEx system / Thomas Floss and Frank Schnütgen

Steps toward targeted insertional mutagenesis with class II transposable elements / Sareina Chiung-Yuan wu ... [et al.]

Targeting integration of the Saccharomyces Ty5 retrotransposon / Troy L. Brady, Clarice L. Schmidt, and Daniel F. Voytas

Site-specific chromosomal integration mediated by [phi]C31 integrase / Annahita Keravala and Michele P. Calos

Triplex-mediated gene modification / Erica B. Schleifman, Joanna Y.Chin, and Peter M. Glazer

Allelic exchange of unmarked mutations in Mycobacterium tuberculosis / Martin S. Pavelka, Jr.

Mycobacterial recombineering Julia C. van Kessel and Graham F. Hatfull

Chromosomal engineering of Clostridium perfringens using group II introns / Phalguni Gupta and Yue Chen.

Notes:

Includes bibliographical references and index.

ISBN:

158829899X

9781588298997

OCLC:

176628259