Microchip-based assay systems : methods and applications / edited by Pierre N. Floriano.

Format:

Book

Contributor:

Floriano, Pierre N.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 385.

Methods in molecular biology ; 385

Language:

English

Subjects (All):

Biochips--Laboratory manuals.

Biochips.

DNA microarrays--Laboratory manuals.

DNA microarrays.

Protein microarrays--Laboratory manuals.

Protein microarrays.

Microchip Analytical Procedures--methods.

Medical Subjects:

Microchip Analytical Procedures--methods.

Genre:

Laboratory manuals.

Physical Description:

xi, 229 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

Microchip-Based Assay Systems explores recent progress in the microelectronics arena, the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world. While the area of microelectronics faces daunting challenges, the field also promises fascinating new advances that may translate into products such as sensors and devices that use nanometer-sized building blocks for real-world applications. The contributors to this volume are part of growing interdisciplinary efforts among pioneers in microelectronics, nanoscience, and health that will continue to create exciting new microchip-based applications across a variety of sectors. In the 16 chapters of this volume, leading scientists present technically precise microchip-based assays for a variety of applications. The protocols detailed in this volume will help foster interest in microchip-based assays by providing readers with all the tools necessary to create their own microchip-based assays targeted to new applications. Certainly, this compilation of methods and protocols will help to expand the scope and accelerate the transition of microchip-based assays from academic and industrial research and development centers to real-world use.

Contents:

Microchip electrophoresis for DNA separation by wire-imprinted microchannels on PMMA substrates / Shu-Hui Chen

Fabrication of porous polymer monoliths in microfluidic chips for selective nucleic acid concentration and purification / Jay A.A. West and Brent C. Satterfield

Rapid electrical lysis of bacterial cells in a microfluidic device / Hsiang-Yu Wang ... [et al.]

On-chip bioassay using immobilized sensing bacteria in three-dimensional microfluidic network / Hirofumi Tani, Koji Maehana, and Tamio Kamidate

Microchip-based enumeration of human white blood cells / Pierre Floriano ... [et al.]

Microchip for the diagnosis of cervical cancer / Anja Gulliksen and Frank Karlsen

DNA microchips toward molecular signatures in cervical cancers / Yick F. Wong ... [et al.]

Impedimetric detection for DNA hybridization within microfluidic biochips / Louise Lingerfelt ... [et al.]

Applications of functional protein microarrays: identifying protein-protein interactions in an array format / Matthew A. Coleman ... [et al.]

A microchip-based assay for interleukin-6 / Nicolaos Christodoulides ... [et al.]

Allergen microarrays for the diagnosis of specific IgE against components of cow milk and hen egg in a multiplex biochip-based immunoassay / Christian Harwanegg, Sabine Hutter, and Reinhard Hiller

Surface plasmon resonance imaging on polypyrrole protein chips: application to streptavidin immobilization and immunodetection / Emilie Mercey ... [et al.]

Protein array-based multiplexed cytokine assays / Cheng C. Wang

Lectin microarrays for glycoprotein analysis / Lara K. Mahal and Kanoelani Pilobello

Interaction of HIV RNA with peptides detected by acoustic shear wave sensor operated in an on-line format / Anil Deisingh ... [et al.]

Microchip-based electrochemical enzyme immunoassays / Madhu Prakash Chatrathi, Greg Collins, and Joseph Wang.

Notes:

Includes bibliographical references and index.

ISBN:

1588295885

9781588295880

9781597454261

1597454265

OCLC:

70327992