Hedgehog signaling protocols / edited by Jamila I. Horabin.

Format:

Book

Contributor:

Horabin, Jamila I.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 397.

Methods in molecular biology ; 397

Language:

English

Subjects (All):

Cellular signal transduction--Research--Methodology.

Cellular signal transduction.

Transcription factors--Research--Methodology.

Transcription factors.

Signal Transduction.

Research.

Methodology.

Medical Subjects:

Signal Transduction.

Physical Description:

xvii, 256 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana ; [Paisley] : [Quantum, distributor], [2007]

Summary:

In both vertebrates and invertebrates. Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, making it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches which are varied and broad in nature, as well as procedures for analyzing a variety of different model systems. Researchers will find this volume to be an invaluable tool as they work towards a deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates. Features: Offers detailed chapters on various experimental design, including anecdotal pointers and notes, Intended for molecular biologists, biochemists, and geneticists.

Contents:

1 Purifying the Hedgehog Protein and its Variants / Darren P. Baker, Frederick R. Taylor, R. Blake Pepinsky 1

2 Application of Sonic Hedgehog to the Developing Chick Limb / Eva Tiecke, Cheryll Tickle 23

3 Manipulation of Hedgehog Signaling in Xenopus by Means of Embryo Microinjection and Application of Chemical Inhibitors / Thomas Hollemann, Emmanuel Tadjuidje, Katja Koebernick, Tomas Pieler 35

4 Isolation of Rat Telencephalic Neural Explants to Assay Shh GABAergic Interneuron Differentiation-Inducing Activity / Rina Mady, Jhumku D. Kohtz 47

5 Genetic Analysis of the Vertebrate Hedgehog-Signaling Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo / Sudipto Roy 55

6 Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems / Maria Kasper, Gerhard Regl, Thomas Eichberger, Anna-Maria Frischauf, Fritz Aberger 67

7 Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry / Kristina Detmer, Ronald E. Garner 79

8 Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional Analysis / Ainhoa Callejo, Luis Quijada, Isabel Guerrero 91

9 Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent in situ Hybridization of Hedgehog Target Genes / Armel Gallet, Pascal P. Therond 105

10 RNAi in the Hedgehog Signaling Pathway: pFRiPE, a Vector for Temporally and Spatially Controlled RNAi in Drosophila / Eric Marois, Suzanne Eaton 115

11 Germline Clone Analysis for Maternally Acting Drosophila Hedgehog Components / Erica M. Selva, Beth E. Stronach 129

12 Clonal Analysis of Hedgehog Signaling in Drosophila Somatic Tissues / Christine M. Bankers, Joan E. Hooper 145

13 GAL4/UAS Targeted Gene Expression for Studying Drosophila Hedgehog Signaling / Denise Busson, Anne-Marie Pret 161

14 Biochemical Fractionation of Drosophila Cells / Melanie Stegman, David Robbins 203

15 Using Immunoprecipitation to Study Protein-Protein Interactions in the Hedgehog-Signaling Pathway / Chao Tong, Jin Jiang 215

16 Sequence Analyses to Study the Evolutionary History and Cis-Regulatory Elements of Hedgehog Genes / Ferenc Muller, Anne-Gaelle Borycki 231.

Notes:

Includes bibliographical references and index.

ISBN:

9781588296924

158829692X

9781597455169

1597455164

OCLC:

123798709