Methods in membrane lipids / edited by Alex M. Dopico.

Format:

Book

Contributor:

Dopico, Alex M.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 400.

Methods in molecular biology ; 400

Language:

English

Subjects (All):

Membrane lipids--Research--Methodology.

Membrane lipids.

Membrane Lipids.

Research.

Methodology.

Medical Subjects:

Membrane Lipids.

Genre:

Laboratory manuals.

Physical Description:

xvi, 621 pages, 4 unnumbered pages of plates : illustrations (some color) ; 26 cm.

Place of Publication:

Totowa, N.J. : Humana ; [Paisley] : [Quantum, distributor], [2007]

Summary:

Methods in Membrane Lipids presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. This volume provides a comprehensive set of techniques for studying membrane lipids, and their interactions with other membrane lipids, with membrane proteins, and with exogenous agents such as anesthetics. The progression of chapters increases in complexity, initially presenting introductory concepts and methods for the study of simple structures and interactions, then moving to the study of more complex structures and interactions, including: behaviors of lipids in water systems and phases; membrane lipid movements and diffusion; properties of lipids in mono and bilayers; complex lipid domains; and membrane-lipid interaction with proteins and drugs. Each chapter is written by an internationally recognized expert in the field. Features: A comprehensive set of methods for studying membrane lipids with a strong biophysical emphasis, Provides comparison of various available techniques including pros and cons as seen by the experts, Each chapter includes an introduction to the particular topic and method.

Contents:

1 A Glance at the Structural and Functional Diversity of Membrane Lipids / Alex M. Dopico, Gabor J. Tigyi 7

2 Membrane Lipid Polymorphism: Relationship to Bilayer Properties and Protein Function / Richard M. Epand 15

3 Acrylodan-Labeled Intestinal Fatty Acid-Binding Protein to Measure Concentrations of Unbound Fatty Acids / Jeffrey R. Simard, Frits Kamp, James A. Hamilton 27

4 Measuring Molecular Order and Orientation Using Coherent Anti-Stokes Raman Scattering Microscopy / Hilde A. Rinia, George W. H. Wurpel, Michiel Muller 45

5 Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-ray Scattering / Stephanie A. Tristram-Nagle 63

6 Nuclear Magnetic Resonance Investigation of Oriented Lipid Membranes / Olivier Soubias, Klaus Gawrisch 77

7 Molecular Dynamics Simulations as a Complement to Nuclear Magnetic Resonance and X-Ray Diffraction Measurements / Scott E. Feller 89

8 Use of Inverse Theory Algorithms in the Analysis of Biomembrane NMR Data / Edward Sternin 103

9 Statistical Thermodynamics Through Computer Simulation to Characterize Phospholipid Interactions in Membranes / Mihaly Mezei, Pal Jedlovszky 127

10 Fluorometric Assay for Detection of Sterol Oxidation in Liposomal Membranes / Parkson Lee-Gau Chong, Michelle Olsher 145

11 Fluorescence Detection of Signs of Sterol Superlattice Formation in Lipid Membranes / Parkson Lee-Gau Chong, Berenice Venegas, Michelle Olsher 159

12 Differential Scanning Calorimetry in the Study of Lipid Phase Transitions in Model and Biological Membranes: Practical Considerations / Ruthven N. A. H. Lewis, David A. Mannock, Ronald N. McElhaney 171

13 Pressure Perturbation Calorimetry / P. D. Heiko Heerklotz 197

14 Fourier Transform Infrared Spectroscopy in the Study of Lipid Phase Transitions in Model and Biological Membranes: Practical Considerations / Ruthven N. A. H. Lewis, Ronald N. McElhaney 207

15 Optical Dynamometry to Study Phase Transitions in Lipid Membranes / Rumiana Dimova, Bernard Pouligny 227

16 Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes / Kellen Brunaldi, Jeffrey R. Simard, Frits Kamp, Charu Rewal, Tanong Asawakarn, Paul O'Shea, James A. Hamilton 237

17 Measurement of Lateral Diffusion Rates in Membranes by Pulsed Magnetic Field Gradient, Magic Angle Spinning-Proton Nuclear Magnetic Resonance / Klaus Gawrisch, Holly C. Gaede 257

18 Using Fluorescence Recovery After Photobleaching to Measure Lipid Diffusion in Membranes / Conrad W. Mullineaux, Helmut Kirchhoff 267

19 Single-Molecule Fluorescence Microscopy to Determine Phospholipid Lateral Diffusion / Michael J. Murcia, Sumit Garg, Christoph A. Naumann 277

20 Modeling 2D and 3D Diffusion / Michael J. Saxton 295

21 Measurement of Water and Solute Permeability by Stopped-Flow Fluorimetry / John C. Mathai, Mark L. Zeidel 323

22 Fluorescence Microscopy to Study Pressure Between Lipids in Giant Unilamellar Vesicles / Anna Celli, Claudia Y. C. Lee, Enrico Gratton 333

23 X-Ray Scattering and Solid-State Deuterium Nuclear Magnetic Resonance Probes of Structural Fluctuations in Lipid Membranes / Horia I. Petrache, Michael F. Brown 341

24 Determination of Lipid Spontaneous Curvature from X-Ray Examinations of Inverted Hexagonal Phases / Michael M. Kozlov 355

25 Shape Analysis of Giant Vesicles With Fluid Phase Coexistence by Laser Scanning Microscopy to Determine Curvature, Bending Elasticity, and Line Tension / Samuel T. Hess, Manasa V. Gudheti, Michael Mlodzianoski, Tobias Baumgart 367

26 Laser Tweezer Deformation of Giant Unilamellar Vesicles / Cory Poole, Wolfgang Losert 389

27 Measurement of Lipid Forces by X-Ray Diffraction and Osmotic Stress / Horia I. Petrache, Daniel Harries, V. Adrian Parsegian 405

28 Micropipet Aspiration for Measuring Elastic Properties of Lipid Bilayers / Marjorie L. Longo, Hung V. Ly 421

29 Langmuir Films to Determine Lateral Surface Pressure on Lipid Segregation / Antonio Cruz, Jesus Perez-Gil 439

30 Detergent and Detergent-Free Methods to Define Lipid Rafts and Caveolae / Rennolds S. Ostrom, Xiaoqiu Liu 459

31 Near-field Scanning Optical Microscopy to Identify Membrane Microdomains / Anatoli Ianoul, Linda J. Johnston 469

32 Fluorescence Microscopy to Study Domains in Supported Lipid Bilayers / Jonathan M. Crane, Lukas K. Tamm 481

33 Fluorescence Resonance Energy Transfer to Characterize Cholesterol-Induced Domains / Luis M. S. Loura, Manuel Prieto 489

34 Lipid Domains in Supported Lipid Bilayer for Atomic Force Microscopy / Wan-Chen Lin, Craig D. Blanchette, Timothy V. Ratto, Marjorie L. Longo 503

35 Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers / Xiao-Min Gong, Carla M. Franzin, Khang Thai, Jinghua Yu, Francesca M. Marassi 515

36 Laurdan Studies of Membrane Lipid-Nicotinic Acetylcholine Receptor Protein Interactions / Silvia S. Antollini, Francisco J. Barrantes 531

37 Single-Molecule Methods for Monitoring Changes in Bilayer Elastic Properties / Olaf S. Andersen, Michael J. Bruno, Haiyan Sun, Roger E. Koeppe II 543

38 Ion-Channel Reconstitution / Francisco J. Morera, Guillermo Vargas, Carlos Gonzalez, Eduardo Rosenmann, Ramon Latorre 571

39 The Use of Differential Scanning Calorimetry to Study Drug-Membrane Interactions / Thomas M. Mavromoustakos 587

40 Atomic Force Microscopy to Study Interacting Forces in Phospholipid Bilayers Containing General Anesthetics / Zoya V. Leonenko, Eric Finot, David T. Cramb 601.

Notes:

Includes bibliographical references and index.

ISBN:

9781588296627

1588296628

9781597455190

1597455199

OCLC:

123798708