Reporter genes : a practical guide / edited by Donald S. Anson.

Format:

Book

Contributor:

Anson, Donald S.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 411.

Methods in molecular biology ; 411

Language:

English

Subjects (All):

Reporter genes.

Mammals--Cytology.

Mammals.

Mammals--Genetics.

Genes, Reporter.

Cytological Techniques.

Mammals--genetics.

Medical Subjects:

Genes, Reporter.

Cytological Techniques.

Mammals--genetics.

Physical Description:

x, 158 pages : illustrations (some color) ; 24 cm + 1 CD-ROM (4 3/4 in.)

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of [beta]-galactosidase in embryos and tissues, immunohistochemical detection of [beta]-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.

Features: Provides practical guides for the use of the reporter genes most commonly used in mammalian systems including [beta]-galactosidase, GFP and luciferase, Easy to follow protocols for the use of reporter genes in various eukaryotic systems, Follows the step-by-step method pioneered by the Methods in Molecular Biology[Trademark] series.

Contents:

Methodologies for staining and visualization of [beta]-galactosidase in mouse embryos and tissues / Siobhan Loughna and Deborah Henderson

Immunohistochemical detection of [beta]-galactosidase or green fluorescent protein on tissue sections / Philip A. Seymour and Maike Sander

Detection of reporter gene expression in murine airways / Maria Limberis, Peter Bell, and James M. Wilson

Three-dimensional analysis of molecular signals with episcopic imaging techniques / Wolfgang J. Weninger and Timothy J. Mohun

Fluorescent proteins for cell biology / George H. Patterson

Detection of GFP during nervous system development in Drosophila melanogaster / Karin Edoff, James S. Dods, and Andrea H. Brand

Autofluorescent proteins for flow cytometry / Charles G. Bailey and John E. J. Rasko

Fluorescent protein reporter systems for single-cell measurements / Steven K. Dower, Eva E. Qwarnstrom, and Endre Kiss-Toth

Subcellular imaging of cancer cells in live mice / Robert M. Hoffman

Noninvasive imaging of molecular events with bioluminescent reporter genes in living subjects / Pritha Ray and Sanjiv Sam Gambhir

Green fluorescent protein as a tracer in chimeric tissues : the power of vapor fixation / Harald Jockusch and Daniel Eberhard.

Notes:

Includes bibliographical references and index.

ISBN:

9781588297396

158829739X

OCLC:

84726587