Measuring gene expression / Matthew B. Avison.

Format:

Book

Author/Creator:

Avison, Matthew B.

Series:

Basics (BIOS Scientific Publishers)

The basics

Language:

English

Subjects (All):

Gene expression.

Gene Expression.

Genetic Techniques.

Medical Subjects:

Gene Expression.

Genetic Techniques.

Physical Description:

x, 324 pages : illustrations ; 25 cm.

Place of Publication:

New York : Taylor & Francis, [2007]

Summary:

In the post-genomic age, much biomedical research looks at when, where, and at what level genes are expressed. Measuring Gene Expression is an all-in-one introduction to the main methods of measuring gene expression, including PCR-based methods, differential display, reporter gene assays, microarrays, and proteomics, as well as a section on RNA isolation and analysis. It provides the information and protocols required to allow the reader to select the most appropriate method to measure gene expression in their experimental system and then interpret the results.

Contents:

Gene expression and its control

Isolation and analysis of RNA

Hybridization-based methods for measuring transcript levels

PCR-based methods for measuring transcript levels

Differential display, subtractive hybridization, amplification suppression, and SAGE techniques for measuring gene expression

Measuring gene expression using reporter gene assays

Analysis of the proteome

Statistical analysis of gene expression data.

Notes:

Includes bibliographical references and index.

ISBN:

0415374723

9780415374729

OCLC:

67817137

Online:

Publisher description