Molecular motors : methods and protocols / Ann O. Sperry.

Format:

Book

Author/Creator:

Sperry, Ann O.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 392.

Methods in molecular biology ; 392

Language:

English

Subjects (All):

Molecular biology.

Molecular Motor Proteins.

Medical Subjects:

Molecular Motor Proteins.

Genre:

Laboratory manuals.

Physical Description:

xi, 247 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana, 2007.

Summary:

Molecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1 ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well. Features: Provides step-by-step instruction, Includes a detailed Notes section, Based on the hands-on experience of the authors.

Contents:

Equilibrium binding of proteins to F-actin / Joseph M. Chalovich

Analysis of calcium/calmodulin regulation of a plant kinesin using co-sedimentation and ATPase assays / Anireddy S.N. Reddy

In vitro and in vivo analysis of microtubule-destabilizing kinesins / Jason Stumpff ... [et al.]

Approaches to kinesin-1 phosphorylation / Gerardo Morfini, Gustavo Pigino, and Scott T. Brady

Protein modification to probe intradynein interactions and in vivo redox state / Ken-ichi Wakabayashi, Miho Sakato, and Stephen M. King

Methods to study the interactions of the dynein light chains and intermediate chains / Kevin W.-H. Lo and K. Kevin Pfister

Identification of motor protein cargo by yeast 2-hybrid and affinity approaches / Yuguo Zhang ... [et al.]

In situ binding assay to detect myosin-1c interactions with hair-cell proteins / Kelli R. Phillips and Janet L. Cyr

Ultrastructural analysis of kenesin-related motor proteins during spermatogenesis / Wan-Xi Yang

In vitro motility system to study the role of motor proteins in receptor-ligand sorting / John W. Murray and Allan W. Wolkoff

Enrichment and disassembly of ectoplasmic specializations in the rat testis / Julian A. Guttman, Kuljeet S. Vaid, and A. Wayne Vogl

Single-molecule observation of rotation of F1-ATPase through microbeads / Takayuki Nichizaka, Kana Mizutani, and Tomoko Masaike

The use of FRET in the analysis of motor protein structure / Andrzej A. Kasprzak

Structure determination of the motor domain of yeast kinesin Kar3 by X-ray crystallography / Hee-Won Park

High-resolution structural analysis of the kinesin-microtubule complex by electron cryo-microscopy / Keiko Hirose and Linda A. Amos

Chemical-genetic inhibition of sensitized mutant unconventional myosins / Ryan L. Karcher ... [et al.]

ISBN:

9781588296658

1588296652

OCLC:

82672211