Immunological tolerance : methods and protocols / edited by Paul J. Fairchild.

Format:

Book

Contributor:

Fairchild, Paul J.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 380.

Methods in molecular biology ; v. 380

Language:

English

Subjects (All):

Immunological tolerance--Laboratory manuals.

Immunological tolerance.

Dendritic cells--Immunology--Laboratory manuals.

Dendritic cells.

Immunosuppression--Laboratory manuals.

Immunosuppression.

Immune Tolerance.

Immunology.

Dendritic Cells--immunology.

Clinical Laboratory Techniques.

Models, Animal.

T-Lymphocytes--immunology.

Medical Subjects:

Immune Tolerance.

Dendritic Cells--immunology.

Clinical Laboratory Techniques.

Models, Animal.

T-Lymphocytes--immunology.

Genre:

Laboratory manuals.

Physical Description:

xiv, 477 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

Immunological Tolerance: Methods and Protocols provides a comprehensive guide to the techniques currently used for culturing and characterizing the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo. The volume is divided into easy to follow sections. The first section is an introduction to immunological tolerance, while the second section discusses the generation and culture of thymic epithelial cells, regulatory T cells and dendritic cells. The next section describes protocols for studying tolerance in vitro while another section explores the study of tolerance in vivo. The final section reviews both proven and novel strategies for the induction of tolerance through mixed chimerism, the adoptive transfer of regulatory T cells, or the administration of biologicals. With meticulous analysis of novel approaches, Immunological Tolerance: Methods and Protocols provides a thorough guide to the field of immunological tolerance.

Features: Provides up-to-date reviews of the literature on essential topics in immunological tolerance, Methods for the generation and culture of critical cell types and approaches to characterization, Describes protocols for the study of tolerance both in vitro and in vivo, Provides insights into novel techniques for tolerance induction.

Contents:

Frontiers of immunological tolerance / Giogrgio Raimondi, Hēth R. Turnquist, and Angus W. Thomson

Part I, Cell types contributing to immunological tolerance

Balancing tolerance and immunity: the role of dendritic cell and t cell subsets / Elena Shklovskaya and Barbara Fazekas de St. Groth

Differentiation of dendritic cell subsets from mouse bone marrow / Ludovica Bruno

Genetic modification of dendritic cells through the directed differentiation of embryonic stem cells / Paul J. Fairchild, Kathleen F. Nolan, and Herman Waldmann

Generation of immunocompetent t cells from embryonic stem cells / Renée F. de Pooter and Juan Carlos Zúñiga-Pflücker

Isolation, expansion, and characterization of human natural and adaptive regulatory t cells / Silvia Gregori ... [et al.]

Derivation, culture, and characterization of thymic epithelial cell lines / Michiyuki Kasai and Toshiaki Mizuochi

Part II, The study of immunological tolerance in vitro

Thymus organogenesis and development of the thymic stroma / Craig S. Nowell, Alison M. Farley, and C. Clare Blackburn

Generation of a tissue-engineered thymic organoid / Fabrizio Vianello and Mark C. Poznansky

Studying t-cell repertoire selection using fetal thymus organ cultures / Philip G. Ashton-Rickardt

Investigating central tolerance with reaggregate thymus organ cultures / Graham Anderson and Eric J. Jenkinson

Estimating thymic function through quantification of t-cell receptor excision circles / Marie-Lise Dion, Rafick-Pierre Sékaly, and Rémi Cheynier

Gene expression profiling of dendritic cells by microarray / Maria Foti, Paola Ricciardi-Carastagnoli, and Francesca Granucci

SAGE analysis of cell types involved in tolerance induction / Kathleen F. Nolan, Stephen P. Cobbold, and Herman Waldmann

Analyzing the physicodynamics of immune cells in a three-dimensional collagen matrix / Peter Reichardt, Frank Gunzer, and Matthias Gunzer

Part III, The study of immunological tolerance in vivo

Etiology of autoimmune disease: how t cells escape self-tolerance / Eli Sercarz and Claudia Raja-Gabaglia

Animal models of spontaneous autoimmune disease: type 1 diabetes in the nonobese diabetic mouse / Nadia Giarratana, Giuseppe Penna, and Luciano Adorini

Antigen-based therapy and immune regulation in experimental autoimmune encephalomyelitis / Mandy J. McGeachy ... [et al.]

Induction and regulation of inflammatory bowel disease in immunodeficient mice by distinct CD4+ t-cell subsets / Kevin J. Maloy

In vivo models for the study of transplantation tolerance / Hannah Stewart, Rommel Ravanan, and Richard Smith

Ectopic transplantation of tissues under the kidney capsule / Nathan J. Robertson, Paul J. Fairchild, and Herman Waldmann

Intravital two-photon imaging of t-cell priming and tolerance in the lymph node / Susanna Celli and Philippe Bousso

Tracing tolerance and immunity in vivo by CFSE-labeling of administered cells / Elizabeth Ingulli

Part IV, Methods for inducing and breaking immunological tolerance

Thymic involution: implications for self-tolerance / Frances T. Hakim and Ronald E. Gress

Inducing mixed chimerism and transplantation tolerance through allogeneic bone marrow transplantation with costimulation blockade / Ines Pree and Thomas Wekerle

Induction of dominant tolerance using monoclonal antibodies / Ana Água-Doce and Luis Graça

Induction of tolerance by adoptive transfer of treg cells / Kanji Nagahama, Eiji Nishimura, and Shimon Sakaguchi

Modulation of the immune response using dendritic cell-derived exosomes / Nicole R. Bianco ... [et al.]

Breaking self-tolerance to tumor-associated antigens by in vivo manipulation of dendritic cells / Ines Mende and Edgar G. Engleman.

Notes:

Includes bibliographical references and index.

ISBN:

1588296520

9781588296528

1597453951

9781597453950

OCLC:

70713511