Yeast gene analysis / edited by Ian Stansfield and Michael J.R. Stark.

Format:

Book

Contributor:

Stansfield, Ian, Dr.

Stark, Michael J. R.

Series:

Methods in microbiology 0580-9517 ; vol. 36.

Methods in microbiology, 0580-9517 ; vol. 36

Language:

English

Subjects (All):

Mycorrhizas--Research--Methodology.

Mycorrhizas.

Microbiology--Technique.

Microbiology.

Microbiological Techniques.

Yeasts.

Medical Subjects:

Microbiological Techniques.

Yeasts.

Physical Description:

xvi, 700 pages, 12 unnumbered pages plates : illustrations ; 25 cm.

Edition:

Second edition.

Place of Publication:

Amsterdam ; Boston : Elsevier/Academic Press, 2007.

Summary:

Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research. Yeast Gene Analysis, Second Edition, edited by two leading experts in the field, focuses on Saccharomyces cerevisiae and represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions.

Originally published shortly after completion of the yeast genome sequence, this new edition covers many of the major genome-wide strategies that have been developed subsequently, for example, microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. However, the basic methods of yeast molecular biology such as strain construction, immunological methods, generating conditional alleles of essential genes and PCR-mediated gene targeting have not been forgotten, and up-to-date procedures for these and many other standard approaches are also included. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.

Key Features: Aimed at both the novice and the experienced yeast researcher, Provides a combination of background information, experimental protocols and technical tips, Includes new chapters on strain construction, genome-wide two-hybrid approaches, use of microarrays for transcript analysis, real-time analysis of chromosome behaviour, FRET, synthetic gene array technology, protein arrays, chemical genomics, yeast prions, RNA gene analysis, mitochondrial gene function analysis, phylogenetic footprinting, discovering human gene function and predicting yeast gene function.

Contents:

2 Yeast Genetics and Strain Construction / I Stansfield, MJR Stark 23

3 Yeast Transformation / D Truong, RD Gietz 45

4 A Guided Tour to PCR-based Genomic Manipulations of S. cerevisiae (PCR-targeting) / CI Maeder, P Maier, M Knop 55

5 Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles / MJR Stark 79

6 Yeast Hybrid Approaches / E Izumchenko, M Wolfson, EA Golemis, IG Serebriiskii 103

7 Array-Based Yeast Two-Hybrid Screening for Protein-Protein Interactions / SV Rajagopala, B Titz, P Uetz 139

8 Reporter Genes and Their Uses in Studying Yeast Gene Expression / T von der Haar, LJ Josse, LJ Byrne 165

9 Transcript Analysis: A Microarray Approach / A Hayes, JI Castrillo, SG Oliver, A Brass, LAH Zeef 189

10 GFP-based Microscopic Approaches for Whole Chromosome Analysis in Yeasts / Q Gao, TU Tanaka, X He 221

11 Immunological Methods / EH Hettema, KR Ayscough 241

12 Measuring the Proximity of Proteins in Living Cells by Fluorescence Resonance Energy Transfer between CFP and YFP / TN Davis, EGD Muller 269

13 Identification, Characterization, and Phenotypic Analysis of Covalently Linked Cell Wall Proteins / FM Klis, P De Groot, S Brul 281

14 Yeast Protein Microarrays / J Ptacek, M Snyder 303

15 Smart Genetic Screens / M Breitenbach, JR Dickinson, P Laun 331

16 High-Throughput Strain Construction and Systematic Synthetic Lethal Screening in Saccharomyces cerevisiae / AHY Tong, C Boone 369

17 Chemical Genomic Tools for Understanding Gene Function and Drug Action / C Nislow, G Giaever 387

18 RNA Gene Analysis / C Saveanu, M Fromont-Racine, A Jacquier 415

19 Analysis of Gene Function of Mitochondria / S Duvezin-Caubet, AS Reichert, W Neupert 445

20 Yeast Prions and Their Analysis In Vivo / MF Tuite, LJ Byrne, L Josse, F Ness, N Koloteva-Levine, B Cox 491

21 Metabolic Control in the Eukaryotic Cell, a Systems Biology Perspective / JI Castrillo, SG Oliver 527

22 Phylogenetic Footprinting / PF Cliften 551

23 Saccharomyces cerevisiae as a Tool for Human Gene Function Discovery / HR Waterham, RJA Wanders 577

24 Bioinformatic Prediction of Yeast Gene Function / I Lee, R Narayanaswamy, EM Marcotte 597

25 Yeast Genetic Strain and Plasmid Collections / K-D Entian, P Kotter 629

26 Yeast Gene Analysis: The Remaining Challenges / MJR Stark, I Stansfield 667.

Notes:

Includes bibliographical references and index.

ISBN:

9780123694782

0123694787

9780123694799

0123694795

OCLC:

150349167