Protein targeting protocols / edited by Mark van der Giezen.

Format:

Book

Contributor:

Van der Giezen, Mark.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 390.

Methods in molecular biology ; 390

Language:

English

Subjects (All):

Protein binding--Research--Laboratory manuals.

Protein binding.

Protein Binding.

Protein binding--Research.

Medical Subjects:

Protein Binding.

Genre:

Laboratory manuals.

Physical Description:

xv, 501 pages : illustrations (some color) ; 24 cm.

Edition:

Second edition.

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

In this highly anticipated update of the extremely successful Protein Targeting Protocols, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Unlike the first edition, however, this volume places emphasis on protein targeting to cellular compartments in both prokaryotic and eukaryotic systems.

Authors provide state-of-the-art information on the fast moving fields of the protein import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting protocols using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting protocol. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting protocols from different systems, including animal, plant, fungal, and protist models, providing insights into the intricate challenges presented by these diverse systems.

Authoritative and state-of-the-art, Protein Targeting Protocols, Second Edition, provides useful techniques that will aid scientists working with a variety of organisms. Thorough and state-of-the-art update of the successful first edition, Emphasis placed on protein targeting to cellular compartments in both prokaryotic and eukaryotic systems, Targeting protocols from animal, plant, fungal, and protist systems, Chapters written by world leaders in the field.

Contents:

Part I Bacterial Systems

1 Localization and Mobility of Bacterial Proteins by Confocal Microscopy and Fluorescence Recovery After Photobleaching / Conrad W. Mullineaux 3

2 Membrane Protein Insertion and Secretion in Bacteria / Jeanine de Keyzer, Martin van der Laan, Arnold J. M. Driessen 17

3 Identification of Autotransporter Proteins Secreted by Type V Secretion Systems in Gram-Negative Bacteria / Yihfen T. Yen, Christos Stathopoulos 33

4 Periplasmic Targeting of Recombinant Proteins in Escherichia coli / Filipe J. Mergulhao, Gabriel A. Monteiro 47

5 In Vitro Analysis of the Bacterial Twin-Arginine-Dependent Protein Export / Michael Moser, Sascha Panahandeh, Eva Holzapfel, Matthias Muller 63

Part II Eukaryotic Systems

6 Nuclear Import Properties of the Sex-Determining Factor SRY / Jade K. Forwood, Gurpreet Kaur, David A. Jans 83

7 The Mitochondrial Machinery for Import of Precursor Proteins / Kipros Gabriel, Nikolaus Pfanner 99

8 A Genetic Method to Identify Mitochondrial Proteins in Living Mammalian Cells / Takeaki Ozawa, Yoshio Umezawa 119

9 In Vitro and In Vivo Methods to Study Protein Import Into Plant Mitochondria / Shashi Bhushan, Pavel F. Pavlov, Charlotta Rudhe, Elzbieta Glaser 131

10 Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa: In Vitro and In Vivo Studies / Panagiotis Papatheodorou, Grazyna Domanska, Joachim Rassow 151

11 A Mitosome Purification Protocol Based on Percoll Density Gradients and Its Use in Validating the Mitosomal Nature of Entamoeba histolytica Mitochondrial Hsp70 / Jorge Tovar, Sian S. E. Cox, Mark van der Giezen 167

12 The Chloroplast Protein Import Machinery: A Review / Friederike Hormann, Jurgen Soll, Bettina Bolter 179

13 Import of Plastid Precursor Proteins Into Pea Chloroplasts / Bettina Bolter, Jurgen Soll 195

14 Protein Targeting in "Secondary" or "Complex" Chloroplasts / Balbir K. Chaal, Beverley R. Green 207

15 Protein Trafficking to the Complex Chloroplasts of Euglena / Rostislav Vacula, Silvia Slavikova, Steven D. Schwartzbach 219

16 Fluorescent Protein Fusions for Protein Localization in Plants / John Runions, Chris Hawes, Smita Kurup 239

17 Genetic Transformation: A Tool to Study Protein Targeting in Diatoms / Peter G. Kroth 257

18 Identification of Proteins Targeted Into the Endoplasmic Reticulum by cDNA Library Screening / Takeaki Ozawa, Yoshio Umezawa 269

19 Trafficking Through the Early Secretory Pathway of Mammalian Cells / Theresa H. Ward 281

20 Studying Protein Export From the Endoplasmic Reticulum in Plants / Sally L. Hanton, Loren A. Matheson, Federica Brandizzi 297

21 Imaging the Golgi Apparatus in Living Mitotic Cells / Nihal Altan-Bonnet 309

22 Evaluating Yeast Biosynthetic Vacuolar Transport / Brian A. Davies, Darren S. Carney, Bruce F. Horazdovsky 329

23 Targeting to Lysosomes in Mammalian Cells: The Biosynthetic and Endocytic Pathways / Ann H. Erickson, Jeffrey P. Bocock 339

24 Monitoring Autophagy in Yeast: The Pho8[Delta]60 Assay / Daniel J. Klionsky 363

25 Protein Targeting to Yeast Peroxisomes / Ida van der Klei, Marten Veenhuis 373

26 Expression, Localization, and Topology of Fluorescently Tagged Plasma Membrane-Targeted Transmembrane Proteins / Jeanne Shepshelovich, Koret Hirschberg 393

27 Intracellular Protein Localization by Immunoelectron Microscopy / Tetsuaki Osafune, Steven D. Schwartzbach 407

28 Separation of Proteins by Blue Native Electrophoresis / Olga Randelj, Joachim Rassow, Christian Motz 417

29 Computational Prediction of Subcellular Localization / Kenta Nakai, Paul Horton 429

30 Phylogenetic Analysis to Uncover Organellar Origins of Nuclear-Encoded Genes / Bernardo J. Foth 467.

Notes:

Includes bibliographical references and index.

ISBN:

9781588297020

1588297020

9781597454667

1597454664

OCLC:

76864448