Peptide characterization and application protocols / edited by Gregg B. Fields.

Format:

Book

Contributor:

Fields, Gregg B.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 386.

Methods in molecular biology ; 386

Language:

English

Subjects (All):

Peptides--Analysis.

Peptides.

Peptides--analysis.

Medical Subjects:

Peptides--analysis.

Physical Description:

xi, 342 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana, 2007.

Summary:

Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. Conformationally constrained peptides are described as potential ligands, substrates, and enzyme inhibitors. Peptides engineered for these purposes are used in the proteolytic profiling of melanoma and in enhancing drug and siRNA delivery. Chapters examining applications for peptides as imaging, anti-aggregatory, and microbial agents are also presented. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.

Features: HPLC analysis and purification of peptides, MS/MS spectra identification of proteins, Proteolytic profiling of the extracellular matrix degradome, Peptide-mediated targeting of liposomes to tumor cells, Peptide-mediated delivery of nucleic acids into mammalian cells.

Contents:

Pt. I. Characterization. HPLC analysis and purification of peptides / Colin T. Mant ... [et al.]

Identification of proteins based on MS/MS spectra and location of posttranslational modifications / Kathryn L. Stone ... [et al.]

Determination of phosphorylated and o-glycosylated sites by chemical targeting (CTID) at ambient temperature / Gary M. Hathaway

Synthesis, biosynthesis, and characterization of transmembrane domains of a g protein-coupled receptor / Fred Naider

Pt. II. Applications. Application of topologically constrained mini-proteins as ligands, substrates, and inhibitors / Janelle L. Lauer-Fields ... [et al.]

Proteolytic profiling of the extracellular matrix degradome / Diane Baronas-Lowell ... [et al.]

[beta]-amyloid protein aggregation / Marcus A. Etienne ... [et al.]

Radiometal-labeled somatostatin analogs for applications in cancer imaging and therapy / Jason S. Lewis and Carolyn J. Anderson

Cell-penetrating proline-rich peptidomimetics / Josep Farrera-Sinfreu ... [et al.]

Peptide-mediated targeting of liposomes to tumor cells / Evonne M. Rezler ... [et al.]

Peptide-mediated delivery of nucleic acids into mammalian cells / Sébastien Deshayes ... [et al.]

Broth microdilution antibacterial assay of peptides / Laszlo Otvos and Mare Cudic

Depsipeptide synthesis / Maciej Stawikowski and Predrag Cudic.

Notes:

Includes bibliographical references and index.

ISBN:

9781588295507

OCLC:

76798707