Cryopreservation and freeze-drying protocols / edited by John G. Day, Glyn N. Stacey.

Format:

Book

Contributor:

Day, John G., 1961-

Stacey, G. (Glyn)

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 368.

Methods in molecular biology ; 368

Language:

English

Subjects (All):

Cryopreservation of organs, tissues, etc--Methods.

Cryopreservation of organs, tissues, etc.

Freeze-drying--Methods.

Freeze-drying.

Cryopreservation--methods.

Freeze Drying--methods.

Medical Subjects:

Cryopreservation--methods.

Freeze Drying--methods.

Physical Description:

xi, 347 pages : illustrations ; 24 cm.

Edition:

Second edition.

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

Cryopreservation and Freeze-Drying Protocols, Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995.

This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources. Features: Background and Explanatory notes for each protocol, Cutting-edge methods for conservation and sustainable use of resources, Methods for lyophilization of proteins, New and novel techniques for preserving biological materials.

Contents:

1 Long-Term Ex Situ Conservation of Biological Resources and the Role of Biological Resource Centers / Glyn N. Stacey, John G. Day 1

2 The Principles of Freeze-Drying / Gerald Adams 15

3 Principles of Cryopreservation / David E. Pegg 39

4 Lyophilization of Proteins / Paul Matejtschuk 59

5 Vacuum-Drying and Cryopreservation of Prokaryotes / Brian J. Tindall 73

6 Freeze-Drying of Yeast Cultures / Chris Bond 99

7 Cryopreservation of Yeast Cultures / Chris Bond 109

8 Freeze-Drying Fungi Using a Shelf Freeze-Drier / C. Shu-hui Tan, Cor W. van Ingen, Joost A. Stalpers 119

9 Cryopreservation and Freeze-Drying of Fungi Employing Centrifugal and Shelf Freeze-Drying / Matthew J. Ryan, David Smith 127

10 Cryopreservation of Microalgae and Cyanobacteria / John G. Day 141

11 Cryopreservation of Plant Cell Suspensions / Brian W. W. Grout 153

12 Cryopreservation of Shoot Tips and Meristems / Erica E. Benson, Keith Harding, Jason W. Johnston 163

13 Cryopreservation of Desiccation-Tolerant Seeds / Hugh W. Pritchard 185

14 Cryopreservation of Fish Sperm / Eugeny Kopeika, Julia Kopeika, Tiantian Zhang 203

15 Cryopreservation of Avian Spermatozoa / Graham J. Wishart 219

16 Cryopreservation of Animal and Human Cell Lines / Christopher B. Morris 227

17 Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use / Suzanne M. Watt, Eric Austin, Sue Armitage 237

18 Cryopreservation of Human Embryonic Stem Cell Lines / Charles J. Hunt, Paula M. Timmons 261

19 Cryopreservation of Primary Animal Cell Cultures / Glyn N. Stacey, Stuart Dowall 271

20 Cryopreservation of Red Blood Cells and Platelets / Andreas Sputtek 283

21 Cryopreservation of Mammalian Semen / Mark R. Curry 303

22 Cryopreservation of Mammalian Oocytes / Sharon J. Paynter, Barry J. Fuller 313

23 Cryopreservation of Mammalian Embryos / Barry J. Fuller, Sharon J. Paynter 325.

Notes:

Includes bibliographical references and index.

ISBN:

1588293777

9781588293770

OCLC:

68705631