Rat jugular vein and carotid artery catheterization for acute survival studies : a practical guide / Angela Heiser ; foreword by John H.K. Liu.

Format:

Book

Author/Creator:

Heiser, Angela.

Language:

English

Subjects (All):

Rats as laboratory animals.

Arterial catheterization.

Intravenous catheterization.

Catheterization--methods.

Rats--anatomy & histology.

Medical Subjects:

Catheterization--methods.

Rats--anatomy & histology.

Genre:

Laboratory manuals.

Physical Description:

xi, 115 pages : illustrations (mostly color) ; 18 x 26 cm

Place of Publication:

New York ; London : Springer, [2007]

Summary:

This manual is a step-by-step method of two basic catheterizations in the laboratory rat. These vascular surgeries are the most widely used procedures in research laboratories around the world from drug discovery application to critical compound administration and blood sampling.

The unique presentation of this manual brings together all aspects of surgery with listed materials, high resolution pictures, and method descriptions for each step. Chapters focus on surgical technique while addressing animal welfare. Comprehensive information on anatomy, catheter materials, applications, and rat biodata are compiled from multiple resources. The book's easy, flip chart style and combination of reference materials will enable any technician to learn these surgeries effectively and efficiently in a single source for the first time. Angela Heiser's extensive experience in performing and training scientists in these surgical procedures in academia and the pharmaceutical industry for over twenty-five years ensures the practicality of the manual.

The field of animal science is highly regulated to ensure humane and ethical care in the use of laboratory animals as required by the Animal Welfare Act. This comprehensive and concise guide will support animal welfare by improving the understanding of important concepts such as sterile handling, anesthetic stages, and health monitoring. Individuals dedicated to applying these mandates through quality training and education will appreciate this contribution as a unique teaching and learning tool.

Contents:

Perioperative Care (Pre-operative care, Intra-operative care, Post-operative care, Pain, Record keeping) 3

Anesthesia (Ketamine cocktail recipe, Dosage chart, Stages of Anesthesia, Reflexes, Rationale for ketamine, Weight considerations) 7

Aseptic Technique 15

Posture and Muscle Tremor 20

Surgeon's Friction Knot 22

Hemostasis Techniques 24

Catheters (Materials, Fill solutions, Stoppers) 26

Anatomy (General dissection, Vessel anatomy, Unilateral carotid occlusion, Vagus) 29

Practice Surgery (PracticePak, Rat model, Cadaver, Non-survival) 34

Instruments 36

Jugular Vein Catheterization 37

Carotid Artery Catheterization 49

Blood Drawing Via Carotid Catheter (Maximum Volumes, Troubleshooting, Administration Volumes) 66

Research Applications 71

Rat Biodata (General, Reproductive/Cardiovascular, Metabolic, Brain, Clinical Chemistry, Spatial Definitions) 76

Latin Terms 83

Resources (Vendors, Websites) 85.

Notes:

Includes bibliographical references (pages 98-109) and index.

ISBN:

0387494146

9780387494142

OCLC:

77795780