Cellular electron microscopy / edited by J. Richard McIntosh.

Format:

Book

Contributor:

McIntosh, J. Richard.

Series:

Methods in cell biology 0091-679x ; v. 79.

Methods in cell biology, 0091-679x ; v. 79

Language:

English

Subjects (All):

Electron microscopy.

Microscopy--Technique.

Microscopy.

Microscopy, Electron--methods.

Medical Subjects:

Microscopy, Electron--methods.

Physical Description:

xxvii, 850 pages, 28 unnumbered pages of plates : illustrations (some color) ; 24 cm.

Place of Publication:

Amsterdam ; Boston, Mass. : Elsevier/Academic Press, [2007]

Summary:

Recent improvements in electron microscopy of cells are bringing cell biologists back to "EM", particularly as they realize the limitations of the light microscopic methods that have been dominating the study of cell structure. This trend is a direct result of the resolving power of the electron microscope, of improved methods for specimen preparation, and of new technologies for three-dimensional imaging and image analysis. EM is beginning to fulfill its promise of linking light microscopy of live cells with atomic resolution studies of isolated macromolecules, with the goal of improving our understanding of living systems at atomic resolution. These new methods will help biologists from many disciplines to apply modern EM to their own work, allowing them to participate in the groundbreaking discoveries that are now being made in this field.

Cellular Electron Microscopy is divided into five sections, each beginning with an introduction by the editor. The sections cover many issues: specimen preparation, 3D imaging, use of frozen-hydrated samples, labeling macromolecules, and technologies for data aquisition and analysis. This book is explicitly designed for new and potential users, not experts in EM, so the chapters focus on practical information, including discussions of why each topic is important and its likely future developments. The work will prove an invaluable resource for anyone in cell biology, as well as those in biophysics, microbiology, neuroscience, microscopy, biochemistry, and genetics.

Contents:

The Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells / J. Dubochet

Cryopreparation Methods for Electron Microscopy of Selected Model Systems / K. McDonald

Cryopreparation Methodology for Plant Cell Biology / M. W. Hess

Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis / T. Muller-Reichert

Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography / E. T. O'Toole

Whole-Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography / J. L. Hoog

Electron Microscopy of Archaea / C. E. Robertson

Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography / B. J. Marsh

Visualization of Membrane-Cytoskeletal Interactions During Plant Cytokinesis / M. S. Otegui

Electron Tomographic Methods for Studying the Chemical Synapse / C. P. Arthur

Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle / B. F. McEwen

Electron Microscopic Analysis of the Leading Edge in Migrating Cells / T. Svitkina

Imaging Actomyosin In Situ / K. A. Taylor

Electron Tomography of Bacterial Chemotaxis Receptor Assemblies / P. Zhang

How to "Read" a Vitreous Section / J. Dubochet

Single-Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction / I. I. Serysheva

Electron Microscopy of Microtubule-Based Cytoskeletal Machinery / A. Hoenger

Reconstructing the Endocytotic Machinery / Y. Cheng

3D Immunolocalization with Plastic Sections / M. K. Morphew

Electron Microscopy Analysis of Viral Morphogenesis / A. Pelchen-Matthews

Electron Tomography of Immunolabeled Cryosections / M. S. Ladinsky

Visualizing Macromolecules with Fluoronanogold: From Photon Microscopy to Electron Tomography / T. Cheutin

Markers for Correlated Light and Electron Microscopy / G. E. Sosinsky

Localizing Specific Elements Bound to Macromolecules by EFTEM / R. D. Leapman

Localization of Protein Complexes by Pattern Recognition / C. Best

The Application of Energy-Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples / J. C. Bouwer

Optimization of Image Collection for Cellular Electron Microscopy / P. Mooney

Future Directions for Camera Systems in Electron Microscopy / N. Xuong

Structure Determination In Situ by Averaging of Tomograms / F. Forster

Methods for Image Segmentation in Cellular Tomography / K. Sandberg

Database Resources for Cellular Electron Microscopy / M. E. Martone.

Notes:

Includes bibliographical references and index.

ISBN:

0123706475

9780123706478

OCLC:

85847813