Glycovirology protocols / edited by Richard J. Sugrue.

Format:

Book

Contributor:

Sugrue, Richard J.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 379.

Methods in molecular biology ; 379

Language:

English

Subjects (All):

Virology--Technique--Laboratory manuals.

Virology.

Molecular biology--Laboratory manuals.

Molecular biology.

Viruses.

Metabolism.

Virology--Technique.

Viruses--Metabolism--Laboratory manuals.

Glycoproteins--physiology.

Molecular Biology--methods.

Virology--methods.

Viruses--metabolism.

Medical Subjects:

Glycoproteins--physiology.

Molecular Biology--methods.

Virology--methods.

Viruses--metabolism.

Genre:

Laboratory manuals.

Physical Description:

x, 205 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins. Among the topics discussed in this volume are M.E. interaction between respiratory syncytial virus and glycosaminoglycans, acute respiratory syndrome coronavirus 3a protein, the C type lectins DC-SIGN and L-SIGN, N-linked glycans, and using 2-dimensional SDS PAGE to analyze the glycosylation heterogeneity of the respiratory syncytial virus fusion protein. Also covered in this volume are uses of monoclonal antibodies and lectins, the spike glycoprotein, secretion of respiratory syncytial virus F protein from insect cells, the expression and characterization of recombinant dengue virus E protein, and cloning, expression, and a functional analysis of patient-derived hepatitis C virus glycoproteins. The information presented in Glycovirology Protocols will provide insight as to how the techniques of glycobiology can be applied in virology and answer most questions that are of interest to the reader.

Features: An introductory overview of viruses and glycosylation, In-depth coverage of a variety of glycoproteins, Chapters written by world-renowned leaders in the field.

Contents:

Viruses and glycosylation : an overview / Richard J. Sugrue

Interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate / Louay K. Hallak, Steven A. Kwilas, and Mark E. Peeples

Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the baculovirus expression system / Sehaam Khan, Mah-Lee Ng, and Yee-Joo Tan

The C type lectins DC-SIGN and L-SIGN : receptors for viral glycoproteins / Pierre-Yves Lozach ... [et al.]

Functional analysis of the N-linked glycans within the fusion protein of respiratory syncytial virus / Ping Li ... [et al.]

Expression and purification of viral glycoproteins using recombinant vaccinia viruses for functional and structural studies / Zhu-Nan Li and David A. Steinhauer

The use of two-dimensional SDS-PAGE to analyze the glycan heterogeneity of the respiratory syncytial virus fusion protein / Terence P. McDonald and Richard J. Sugrue

The use of monoclonal antibodies and lectins to identify changes in viral glycoproteins that are influenced by glycosylation : the case of respiratory syncytial virus attachment (G) glycoprotein / Joanna Rawling and José A. Melero

Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS-CoV / Shuo Shen, Timothy H.P. Tan, and Yee-Joo Tan

Analysis of glycoproteins of viruses in the family bunyaviridae / Xiaohong Shi and Richard M. Elliott

Secretion of respiratory syncytial virus fusion protein from insect cells using the baculovirus expression system / Boon-Huan Tan, Gaie Brown, and Richard J. Sugrue

Characterization of the dengue virus envelope glycoprotein expressed in Pichia pastoris / Boon-Huan Tan, Jian Lin Fu, and Richard J. Sugrue

Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins / Alexander W. Tarr ... [et al.].

Notes:

Includes bibliographical references and index.

ISBN:

1588295907

9781588295903

1597453935

9781597453936

OCLC:

70713504