Protein phosphatase protocols / edited by Greg Moorhead.

Format:

Book

Contributor:

Moorhead, Greg.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 365.

Methods in molecular biology ; 365

Language:

English

Subjects (All):

Phosphoprotein phosphatases--Laboratory manuals.

Phosphoprotein phosphatases.

Phosphoprotein Phosphatases--analysis.

Phosphoprotein Phosphatases--isolation & purification.

Medical Subjects:

Phosphoprotein Phosphatases--analysis.

Phosphoprotein Phosphatases--isolation & purification.

Genre:

Laboratory manuals.

Physical Description:

xv, 387 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics. Included protocols utilize several model organisms, including yeast, Drosophila, and various plant and human cells. Protein Phosphatase Protocols provides a concise introduction to protein phosphatase research, with particular attention to phosphatase families. Assays for identification, quantification, purification, and functional characterization are presented throughout in specific detail.

Contents:

Analysis of protein phosphatases / Shirish Shenolikar

A brief introduction to the protein phosphatase families / Tomas Mustelin

Small molecule inhibitors of Ser/thr protein phosphatases / Mark Swingle, Li Ni, and Richard E. Honkanen

Synthesis and use of the protein phosphatase affinity matrices microcystin- and microcystin-biotin-sepharose / Greg Moorhead, Timothy A.J. Haystead, and Carol MacKintosh

Utilizing protein phosphatase inhibitors to define PP2A as a regulator of ataxia-telangiectasia mutated (ATM) / Aaron A. Goodarzi ... [et al.]

An automated, fluorescence-based method for continuous assay of PP2A activity / Adam M. Wegner ... [et al.]

An in vivo assay to quantify stable protein phosphatase 2A (PP2A) heterotrimeric species / Matthew S. Gentry, Richard L. Hallberg, and David C. Pallas

Mutagenesis and expression of the scaffolding Aa and Ab subunits of PP2A / Ralf Ruediger, Jin Zhou, and Gernot Walter

Isolation and characterization of PP2A holoenzymes containing FLAG-tagged B subunits / Deanna G. Adams and Brian E. Wadzinski

Purification of PP2A holoenzymes by sequential immunoprecipitation with anti-peptide antibodies / Gernot Walter, Jin Zhou, and Ralf Ruediger

Purification of PP2Ac from bovine heart / Hue T. Tran ... [et al.]

Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions / Laura Trinkle-Mulcahy ... [et al.]

Yeast two-hybrid screens to identify drosophila PP1-binding proteins / Daimark Bennett and Luke Alphey

Identification of cellular protein phosphatase-1 regulators / David W. Roadcap, Matthew H. Brush, and Shirish Shenolikar

Assay for 3-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2 / Masumi Eto and David L. Brautigan

Phosphorylation of the protein phosphatase type 1 inhibitor protein, CPI-17, by protein kinase C / Michael P. Walsh ... [et al.]

Purification of smooth muscle myosin phosphatase using a thiophosphorylated myosin light chain-affinity resin / Meredith Borman and Justin MacDonald

Proteins interacting with saccharomyces cerevisiae type 1 protein phosphatase catalytic subunit identified by single-step affinity purification and mass spectrometry / Edmund P. Walsh ... [et al.]

Expression of protein histidine phosphatase in Escherichia coli, purification, and determination of enzyme activity / Nicole Bäumer .. [et al.]

The use of RNA interference to analyze protein phosphatase function in mammalian cells / Iain Fraser ... [et al.]

Recognition of a PP2C interaction motif in several plant protein kinases / Niranjan Chakraborty, Masaru Ohta, and Jian-Kang Zhu

The use of yeast genetic tools to define biological roles of novel protein phosphatases / Joaquín Ariño ... [et al.]

Targeting of PP2C in budding yeast / Irene M. Ota and James Mapes

Phosphatase targets in TOR signaling / Estela Jacinto

Functional characterization of the small CTD phosphatases (SCPs) / Michele Yeo and Patrick S. Lin

Genome-scale discovery and characterization of class-specific protein sequences / David Kerk

Yeast substrate-trapping system for isolating substrates of protein tyrosine phosphatases / Masahide Fukada and Masaharu Noda.

Notes:

Includes bibliographical references and index.

ISBN:

158829711X

9781588297112

159745267X

9781597452670

OCLC:

64585731