Protocols for nucleic acid analysis by nonradioactive probes.

Format:

Book

Contributor:

Hilario, Elena, 1966-

Mackay, John, 1969-

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 353.

Methods in molecular biology, 1064-3745 ; 353

Language:

English

Subjects (All):

Nucleic acid probes.

Nucleic acids--Analysis.

Nucleic acids.

DNA--analysis.

DNA Probes.

Molecular Probe Techniques.

Nucleic Acid Amplification Techniques.

Medical Subjects:

DNA--analysis.

DNA Probes.

Molecular Probe Techniques.

Nucleic Acid Amplification Techniques.

Physical Description:

x, 321 : illustrations ; 24 cm.

Edition:

Second edition / edited by Elena Hilario, John Mackay.

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

Protocols for Nucleic Acid Analysis by Nonradioactive Probes, Second Edition, provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular Biology[Trademark] series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Features: Readily reproducible protocols for the analysis of nucleic acids by nonradioactive methods, New fluorescent techniques such as Real Time PCR and microarrays, Follow step-by-step instructions that ensure successful results, Master tricks of the trade, troubleshoot, and avoid known pitfalls.

Contents:

Genomic DNA isolation from different biological materials / Duckchul Park

Extraction of plant RNA / Elspeth MacRae

Overview of hybridization and detection techniques / Elena Hilario

Checkerboard DNA-DNA hybridization technology : using digoxigenin detection / Lisa S. Gellen, Glenn M. Wall-Manning, and Chris H. Sissons

Nonradioactive northern and southern analyses from plant samples / Christoph Peterhaensel, Dagmar Weier, and Thomas Lahaye

Screening a BAC library with nonradioactive overlapping oligonucleotide (overgo) probes / Elena Hilario, Tiffany F. Bennell, and Erik Rikkerink

Direct in-gel hybridization of DNA with digoxigenin-labeled probes / Saeed A. Khan and Mohamed S. Nawaz

In situ hybridization of termite microbes / Shigeharu Moriya ... [et al.]

RNA electrophoretic mobility shift assay using a fluorescent DNA sequencer / Yukinori Eguchi

Comparative quantitation of mRNA expression in the central nervous system using fluorescence in situ hybridization / Darren J. Day, Eli M. Mrkusich, and John H. Miller

Visualization of gene expression by fluorescent multiplex reverse transcriptase-PCR amplification / María Rosa Ponce ... [et al.]

Fluorescence in situ hybridization for the identification of environmental microbes / Annelie Pernthaler and Jakob Pernthaler

Introduction to kinetic ("real-time") PCR / John Mackay

Validation of short interfering RNA knockdowns by quantitative real-time PCR / Sukru Tuzmen, Jeff Kiefer, and Spyro Mousses

Real-time quantitative PCR as an alternative to southern blot or fluorescence in situ hybridization for detection of gene-copy number changes / Jasmien Hoebeeck, Frank Speleman, and Jo Vandesompele

Design and work-up of a new molecular diagnostic assay based on real-time PCR / Harald H. Kessler

Real-time PCR fluorescent chemistries / John Mackay and Olfert Landt

Microarrays : an overview / Norman H. Lee and Alexander I. Saeed

Oligonucleotide microarrays for the study of coastal microbial communities / Gaspar Taroncher-Oldenburg and Bess B. Ward.

Notes:

Includes bibliographical references and index.

ISBN:

1588294307

9781588294302

1597452297

9781597452298

OCLC:

63680478