Gene cloning : [principles and applications] / Julia Lodge, Pete Lund & Steve Minchin.

Format:

Book

Author/Creator:

Lodge, Julia.

Contributor:

Lund, Peter A.

Minchin, Steve.

Language:

English

Subjects (All):

Molecular cloning.

Cloning, Molecular.

Gene Library.

Genomics--methods.

Medical Subjects:

Cloning, Molecular.

Gene Library.

Genomics--methods.

Physical Description:

ix, 462 pages : illustrations ; 25 cm

Place of Publication:

New York ; Abingdon [England] : Taylor & Francis Group, [2007]

Summary:

Cloning genes successfully underlies the majority of our knowledge in molecular and cellular biology, but the approaches to doing it are so numerous and diverse, that undergraduates (and graduates) can easily be overwhelmed. Gene Cloning gives an overview of the techniques available to clone genes and how they can be used effectively in both pure and applied research. It shows how cloning genes is an integral part of genomics and relevant in the postgenomic age, being necessary for the isolation, identification, sequencing, and analysis of individual genes, gene families, and entire genomes, and for experimentally testing predictions of gene regulation and function made through bioinformatics. Also covered are applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture. There are learning outcomes at the start of each chapter, which include both factual knowledge and how to apply it, and questions throughout the text to allow readers to test their understanding as they go along. Where possible, examples are from well-known topics with medical relevance. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource for biological and biomedical science courses.

Contents:

Introduction

Genome organization

Key tools for gene cloning

Gene identification and DNA libraries

Screening DNA libraries

Further routes to gene identification

Sequencing DNA

Bioinformatics

Production of proteins from cloned genes

Gene cloning in the functional analysis of proteins

The analysis of the regulation of gene expression

The production and uses of transgenic organisms

Forensic and medical applications.

Notes:

Subtitle from cover.

Includes bibliographical references and index.

ISBN:

0748765344

9780748765348

OCLC:

70164475