In vitro fertilization : a practical approach / edited by David K. Gardner.

Format:

Book

Contributor:

Gardner, David K.

Language:

English

Subjects (All):

Fertilization in vitro, Human.

Fertilization in Vitro.

Reproductive Techniques, Assisted.

Medical Subjects:

Fertilization in Vitro.

Reproductive Techniques, Assisted.

Physical Description:

xvii, 507 pages : illustrations ; 24 cm

Place of Publication:

New York : Informa Healthcare, [2007]

Summary:

Supplying a comprehensive and hands-on approach to in vitro fertilization (IVF), this source presents established state-of-the-art procedures and techniques, as well as the most current research in the field. Expert contributors also discuss the history of IVF and the potential of future research.

Offering essential information for reproductive endocrinologists, practitioners in IVF laboratories, and embryologists, this source guides readers through every step of the process, from patient pre-treatment to monitoring of outcomes...familiarizes readers with key procedures in ovarian stimulation, oocyte retrieval, assessment of gametes and subsequent fertilization, and micromanipulation procedures such as ICSI and embryo biopsy...reviews embryo culture, preimplantation genetic diagnosis, and quality management for optimal IVF outcomes...and considers methods of embryo selection and cryobiology, along with practical aspects of transfer techniques and factors influencing implantation.

Contents:

1 In Vitro Fertilization: The First Three Decades / Jean Cohen, Howard W. Jones, Jr. 1

The History of the Past 1

1950-1978 2

1978-1982 4

1982-1992 6

1992-the Present 12

2 Evaluation and Preparation of the Infertile Couple for In Vitro Fertilization / David R. Meldrum 17

Day 3 Follicle-Stimulating Hormone 17

Antral Follicle Count 18

Clomiphene Citrate Challenge Test 18

Polycystic Ovarian Disease 19

Semen Analysis with White Blood Cells Stain and Culture 19

Strict Morphology 20

Antisperm Antibodies 20

Sperm Penetration Assay 20

Sperm Chromatin Structure Assay 21

Chlamydia 21

Trial Transfer 21

Uterine and Tubal Abnormalities 22

HIV and Hepatitis 22

Endometriosis 22

Diethylstilbesier (DES) Exposure 23

Uterine Fibroids 23

Sexual Dysfunction 23

Personal Habits 23

Psycho-Social Aspects 24

General Health 24

3 Testing Ovarian Reserve / James P. Toner 29

Physiology of Ovarian Reserve 30

Original Understandings 31

Refined Understandings 32

Controversies 36

4 Basics of Ovarian Stimulation / David L. Healy, Sue Breheny, Vivien MacLachlan, Gordon Baker 43

Human Ovarian Physiology 44

Clinical Reproductive Endocrinology 47

Polycystic Ovarian Syndrome 53

Ovarian Hyperstimulation Syndrome 55

Poor Responders 57

Oral Contraceptive Pretreatment 58

Ovarian Stimulation and Ovarian Cancer 58

5 Gonadotropin-Releasing Hormone-Antagonist in Human In Vitro Fertilization / F. Olivennes 67

Phase II Dose-Finding Studies 68

Phase III Randomized Controlled Trials and Open Studies 70

GnRH-nt in Mild Stimulation 73

Remaining Questions on GnRH-nt 76

Indications 77

Poor Responders 78

6 Ultrasonography in In Vitro Fertilization / Roger A. Pierson 91

Ovarian Assessment 92

Ultrasound-Guided Oocyte Retrieval 96

Assessment of the Endometrium at Embryo Transfer 98

Ultrasound-Guided Embryo Transfer 104

7 Oocyte Retrieval / Matts Wikland 117

Technical Aspects 119

Complications 123

8 In Vitro Maturation of Oocytes / Hananel E. G. Holzer, Ri-Cheng Chian, Ezgi Demirtas, Hanadi Ba-Akdah, Seang Lin Tan 127

Oocyte Maturation In Vivo and In Vitro 128

In Vitro Maturation of Oocytes in Infertility Treatments 131

Outline of an IVM Treatment Cycle 136

IVM Treatment Outcome 138

Conclusions and Future Considerations 140

9 Sperm Preparation for IVF and ICSI / Nancy L. Bossert, Christopher J. De Jonge 147

Sperm Collection 148

Sperm Preparation Methods 149

Post-separation Treatment of Spermatozoa 156

10 Assisted Fertilization / Andre Van Steirteghem 161

Intracytoplasmic Sperm Injection 162

Outcome and Children's Health 172

11 Analysis of Fertilization / Jan Tesarik, Carmen Mendoza 183

Biological Basis of Fertilization 184

Specific Features of Fertilization in Assisted Reproduction 188

Normal vs. Abnormal Fertilization 189

Predictive Potential of Zygote Noninvasive Evaluation 190

Etiology and Diagnosis of Abnormal Fertilization 192

Clinical Management of Fertilization Abnormalities 193

12 Embryo Development and Assessment of Viability / Thomas Ebner 199

The Follicle 200

The Oocyte 200

The Zygote 203

The Cleaving Embryo 205

Embryo Metabolism 209

The Blastocyst 211

13 Embryo Culture Systems / David K. Gardner, Michelle Lane 221

Composition of Embryo Culture Media 225

Culture System 248

What Stage Should the Human Embryo Be Transferred? 254

Embryo Culture Protocol 259

Quality Control 261

14 Micromanipulation as a Clinical Tool / Jacques Cohen 283

Gamete Micromanipulation 284

Post-Fertilization 294

Embryo Biopsy 302

Moral, Ethical, and Political Considerations 305

15 Preimplantation Genetic Diagnosis / Mandy G. Katz-Jaffe 313

Source of Genetic Material 314

PGD for Monogenic Disorders 317

Chromosomal Aneuploidy Screening 320

16 Oocyte and Embryo Cryopreservation / Gary D. Smith, Joyce Fioravanti 331

History of Mammalian Gamete/Embryo Cryobiology 331

Principles of Cryopreservation 333

Protocols 334

Troubleshooting and a Successful Cryopreservation Program 356

Future of Gamete/Embryo Cryobiology 358

17 Quality Assurance and Quality Control in the In Vitro Fertilization Laboratory / Karen L. Martin 365

What Are QA and QC? 366

Why Employ QA and QC Schemes in the IVF Laboratory? 366

What Comprises a QC Program? 368

What Comprises a QA Program? 376

International Standards of QA and QC 378

18 Oocyte Donation: State of the Art / Anne Z. Steiner, Richard J. Paulson 381

History 381

Indications 382

Factors Influencing Success 382

Screening of Donors 384

Screening of Recipients 384

Practice Cycle 385

Ovarian Stimulation 386

Donor Safety 387

Endometrial Preparation 388

Embryo Transfer 390

Pregnancy Outcomes 391

Infant Outcomes 392

Advanced Reproductive Age 392

19 Uterine Receptivity / Peter A. W. Rogers 403

Animal Studies and Current Concepts on Uterine Receptivity 404

Theoretical Calculations of Human Uterine Receptivity 406

The Impact of COH on the Endocrine Requirements for Uterine Receptivity 410

The Search for Markers of Uterine Receptivity 416

Age-Related Changes in Uterine Receptivity 423

20 Embryo Transfer Technique / Ragaa Mansour 435

Potential Negative Factors Associated with the ET Technique 436

Suggestions for Optimizing the Technique of ET 438

Description of an ET Procedure 450

Luteal Phase Support 451

21 Monitoring In Vitro Fertilization Outcome / Alastair G. Sutcliffe 465

Why Monitor IVF Outcome? 465

What Are the Problems Known to have Occurred with Outcome Studies? 467

Why Monitor: What Can Go Wrong if the Data Are Misunderstood or Misinterpreted? 467

What Is Known From the Current Literature as an Alternative? 468

Evaluation of the Major Published Studies 469

Physical Assessments Other Than for Congenital Anomalies 478

Genomic Imprinting-the ARTID Study 480.

Notes:

Includes bibliographical references and index.

ISBN:

0849333350

OCLC:

70829695

Publisher Number:

9780849333354

Online:

Publisher description