Metabolomics : methods and protocols / edited by Wolfram Weckwerth.

Format:

Book

Contributor:

Weckwerth, Wolfram.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 358.

Methods in molecular biology, 1064-3745 ; 358

Language:

English

Subjects (All):

Metabolism--Laboratory manuals.

Metabolism.

Metabolism--physiology.

Mass Spectrometry--methods.

Magnetic Resonance Spectroscopy--methods.

Medical Subjects:

Metabolism--physiology.

Mass Spectrometry--methods.

Magnetic Resonance Spectroscopy--methods.

Genre:

Laboratory manuals.

Physical Description:

xii, 293 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2007]

Summary:

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC-separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols.

Features: GC-MS techniques and application to real-world samples, Integration of GC-MS metabolite profiling with protein and transcript profiling, Headspace trapping of volatile compounds, Data integration, biostatistics, and multivariate data mining, Electrochemical detection of metabolites, Pathway analysis as a roadmap for understanding metabolism.

Contents:

Metabolite profiling in blood plasma / Oliver Fiehn and Tobias Kind

Nonsupervised construction and application of mass spectral and retention time index libraries from time-of-flight gas chromatography-mass spectrometry metabolite profiles / Alexander Erban ... [et al.]

Metabolomic profiling of natural volatiles: headspace trapping: GC/MS / Yury M. Tikunov, Francel W.A. Verstappen, and Robert D. Hall

Integrative profiling of metabolites and proteins: improving pattern recognition and biomarker selection for systems level approaches / Katja Morgenthal ... [et al.]

Integrating profiling data: using linear correlation to reveal coregulation of transcript and metabolites / Ewa Urbanczyk-Wochniak, Lothar Willmitzer, and Alisdair R. Fernie

Visualization and analysis of molecular data / Matthias Scholz and Joachim Selbig

A gentle guide to the analysis of metabolomic data / Ralf Steuer ... [et al.]

Capillary electrophoresis-mass spectrometry for metabolomics / Tomoyoshi Soga

Application of liquid chromatography-mass spectrometry analysis in metabolomics: reversed-phase monolithic capillary chromatography and hydrophilic chromatography coupled to electrospray ionization-mass spectrometry / Vladimir V. Tolstikov, Oliver Fiehn, and Nobuo Tanaka

High-performance liquid chromatography separations coupled with coulometric electrode array detectors: a unique approach to metabolomics / Bruce S. Kristal ... [et al.]

Determination of metabolic flux ratios from 13C-experiments and gas chromatography-mass spectrometry data: protocol and principles / Annik Nanchen, Tobias Fuhrer, and Uwe Sauer

Understanding the roadmap of metabolism by pathway analysis / Stefan Schuster, Axel von Kamp, and Mikhail Pachkov

Revealing the metabolome of animal tissues using 1H nuclear magnetic resonance spectroscopy / Mark R. Viant

Nuclear magnetic resonance metabonomics: methods for drug discovery and development / Karl-Heinz Ott and Nelly Aranibar

Practical aspects of uniform stable isotope labeling of higher plants for heteronuclear NMR-based metabolomics / Jun Kikuchi and Takashi Hirayama.

Notes:

Includes bibliographical references and index.

ISBN:

1588295613

OCLC:

64444091

Publisher Number:

9781588295613