Integrated genomics : a discovery-based laboratory course / Guy A. Caldwell, Shelli N. Williams, and Kim A. Caldwell.

Format:

Book

Author/Creator:

Caldwell, Guy A.

Contributor:

Williams, Shelli N.

Caldwell, Kim A.

Language:

English

Subjects (All):

Genomics--Laboratory manuals.

Genomics.

Diagnosis, Laboratory.

Molecular biology--Technique.

Molecular biology.

Genetic Techniques.

Medical Subjects:

Genomics.

Genetic Techniques.

Genre:

Laboratory manuals.

Physical Description:

xx, 225 pages : illustrations ; 29 cm

Place of Publication:

Chichester ; Hoboken, NJ : Wiley, [2006]

Summary:

Integrated Genomics: A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols and a basic experimental design, this text offers experience with three different model systems. Students will become familiar with the simplicity and power of single-celled organisms, E.coli and S.cerevisiae, as they search for genes that interact and function within the nematode C.elegans. They will also begin to explore the wealth of bioinformatics data available on the Internet via numerous exercises incorporated throughout the book.

Using simple and inexpensive techniques, the concluding chapters enable students to examine the phenotypic consequences of reducing gene function within C.elegans. The authors have included a range of alternative experiments that offer flexibility in determining the end date or goal of the laboratory, as well as working within the available budget and resources of most classroom environments.

Key Features: Provides training with multiple inexpensive and popular model organisms including C.elegans and S.cerevisiae, Adopts a student-tested, discovery-based format, Integrates basic bioinformatics within an experimental context, Inclusive of modern molecular genomic/proteomic technologies such as the yeast two-hybrid system and RNAi, Accompanying website includes easy access to instructional materials and experimental reagents including all strains and plasmids used in the course. An invaluable text for advanced undergraduate and postgraduate students studying cell and molecular biology, genetics, microbiology, biochesmistry, proteomics, genomics and bioinformatics.

Contents:

Introduction to basic laboratory genetics

Gene expression analysis using transgenic animals

Creation and testing of transgenic yeast for use in protein-protein interaction screening

Yeast two-hybrid screening

Isolation and identification of interacting proteins

Using bioinformatics in modern science

Generation of an RNAi vector

RNA-mediated interference by bacterial feeding.

Notes:

Includes bibliographical references and index.

ISBN:

0470095016

0470095024

OCLC:

67870925

Publisher Number:

9780470095010

9780470095027