Nucleic acid testing for human disease / edited by Attila Lorincz.

Format:

Book

Contributor:

Lorincz, Attila T.

Language:

English

Subjects (All):

Nucleic acids--Diagnostic use.

Nucleic acids.

Molecular Diagnostic Techniques.

Nucleic Acids--diagnostic use.

Genetic Techniques.

Clinical Laboratory Techniques.

Medical Subjects:

Molecular Diagnostic Techniques.

Nucleic Acids--diagnostic use.

Genetic Techniques.

Clinical Laboratory Techniques.

Physical Description:

470 pages, 8 unnumbered pages of plates : illustrations (partly color) ; 27 cm

Place of Publication:

Boca Raton, FL : CRC Taylor & Francis, 2006.

Summary:

A grab-bag of highly diverse methods, nucleic acid tests (NATS) are a sophisticated and remarkably important medical advance that has grown from relative obscurity to a major part of the repertoire of most clinical testing laboratories. Tens of millions of routine diagnostic NATs are now performed, with millions more conducted in the research setting. NATs may well become the predominant molecular method of testing and measuring human health in a matter of a few decades. Bringing together experts from academia, clinical testing laboratories, and industry, Nucleic Acid Testing for Human Disease describes NAT technologies and their clinical applications.

The book details the myriad techniques used and provides a deeper understanding of how the tests are interpreted as well as the "do's and don'ts" of clinical use. The first section includes technique-oriented chapters on target and signal amplification-based NAT procedures, microarrays, bead-based multiplex assays, in situ hybridization, SNP techniques, RNA expression profiling, and laboratory issues, including the pressing need for proper validation of tests intended for routine diagnostic use. The second section focuses on specific applications of NATs to human disease diagnosis and treatment and runs the gamut from viral, bacterial, fungal, and protozoal detection to human genetic exploration by SNP determinations, patterns of RNA expression, and the growing relevance of epigenetic changes such as methylation. Rounding out the book's coverage, two chapters discuss future directions, one from an academic research perspective and one from the viewpoint of the clinical laboratory director.

Good medicine starts with accurate diagnosis. It is now abundantly clear that favorable outcomes in medicine are tied to robust NAT tests. A comprehensive set of papers by leading researchers in the field, with more than 90 figures and 35 tables, this book sheds light on the current and future role of NATs as exciting tools to further improvement of the human condition.

Contents:

Part I Nucleic Acid Diagnostic Technology

Chapter 1 Target Amplification-Based Techniques / Antoinette A.T.P. Brink, Peter J.F. Snijders, Chris J.L.M. Meijer 3

Chapter 2 Signal Amplification-Based Techniques / David A. Hendricks, Lorraine Comanor 19

Chapter 3 Microarrays: Human Disease Detection and Monitoring / Janet A. Warrington, Thomas B. Broudy 65

Chapter 4 Bead-Based Flow Systems: From Centralized Laboratories to Genetic Testing in the Field / Darrell P. Chandler 93

Chapter 5 In Situ Hybridization / Xiang Qian, Ricardo V. Lloyd 113

Chapter 6 Detection Techniques for Single Nucleotide Polymorphisms / W. Mathias Howell, Johan Stenberg, Chatarina Larsson, Mats Nilsson, Ulf Landegren 139

Chapter 7 RNA Expression Profiling / Payman Hanifi-Moghaddam, Curt W. Burger, Theo J.M. Helmerhorst, Leen J. Blok 171

Chapter 8 Evolution of the Molecular Microbiology Laboratory / Gary W. Procop, Belinda Yen-Lieberman 189

Part II Disease Applications of Nucleic Acid Tests

Chapter 9 Bacterial Sexually Transmitted Diseases / Julius Schachter, Stephen A. Morse 211

Chapter 10 Sexually Transmissible Viral Pathogens: Human Papillomaviruses and Herpes Simplex Viruses / Attila T. Lorincz, Jennifer S. Smith 243

Chapter 11 Blood-Borne Viruses in Clinical and Diagnostic Virology / Hubert G.M. Niesters, Martin Schutten, Elisabeth Puchhammer-Stockl 275

Chapter 12 Molecular Methods for the Diagnosis of Fungal Infections / Adam M. Bressler, Christine J. Morrison 301

Chapter 13 Molecular Diagnostic Approaches in Infectious Disease / Leonard F. Peruski, Jr., Anne Harwood Peruski 331

Chapter 14 Cancer Detection and Prognosis / Santiago Ropero, Manel Esteller 353

Chapter 15 Common Inherited Genetic Disorders / Madhuri Hegde, Sue Richards 373

Chapter 16 Future Perspectives on Nucleic Acid Testing / Larry J. Kricka, Paolo Fortina 413

Chapter 17 Bridging the Gap between Analytical and Clinical Validation / Mel Krajden 435.

Notes:

Includes bibliographical references and index.

ISBN:

157444543X

OCLC:

62782255

Publisher Number:

9781574445435

Online:

Publisher description