Dendrimers in medicine and biotechnology : new molecular tools / U. Boas, J.B. Christensen, P.M.H. Heegaard.

Format:

Book

Author/Creator:

Boas, U.

Contributor:

Christensen, J. B. (Jørn Bolstad)

Heegaard, P. M. H.

Language:

English

Subjects (All):

Molecular structure.

Dendrimers.

Biotechnology.

Physical Description:

ix, 179 pages : illustrations ; 24 cm

Place of Publication:

Cambridge : RSC Publishing, [2006]

Summary:

Dendrimers are a new class of macromolecule increasingly used in the fields of synthetic organic chemistry, biology, medicine and biotechnology. Dendrimers in Medicine and Biotechnology: New Molecular Tools looks at this exciting and rapidly growing area of science. Using an interdisciplinary approach with particular emphasis on biological applications, the book discusses the relationship between the dendrimer molecular motif and its biological properties.

A general introduction to the subject of dendrimers, including definitions of terms and symbols, is provided. Subsequent sections discuss topics including dendrimers in biological systems, dendrimers as drug delivery devices, dendrimers in diagnostics and dendrimer drugs. Throughout the book examples from current research are also provided. This book will appeal to a wide range of scientists, including non specialists who require an introduction to dendrimers, as well as those wishing to know more about the application of dendrimers in the field of biology and medicine.

Contents:

Chapter 1 Dendrimers: Design, Synthesis and Chemical Properties 1

1.2 Terms and Nomenclature in Dendrimer Chemistry 3

1.3 Dendrimer Design 7

1.4 Dendrimer Synthesis 8

1.5 Physicochemical Properties of Dendrimers 16

Chapter 2 Properties of Dendrimers in Biological Systems 28

2.1 Significance of Multivalent Binding in Biological Interactions 28

2.1.1 Dendritic Effect 29

2.1.2 Carbohydrate Ligands 30

2.2 Biocompatibility of Dendrimers 32

2.3 In Vitro Cytotoxicity of Dendrimers 32

2.4 In Vivo Cytotoxicity of Dendrimers 43

2.5 Biopermeability of Dendrimers 46

2.6 Biodistribution of Dendrimers 55

2.7 Immunogenicity of Dendrimers 57

Chapter 3 Dendrimers as Drug Delivery Devices 62

3.2 Dendrimer Hosts 63

3.2.1 Dendrimer Hosts: Non-Specific Interactions with the Dendrimer Core 64

3.2.2 Dendritic Boxes or Topological Trapping of Guests 70

3.2.3 Dendrimer Hosts: Specific Interactions with the Dendrimer Core 71

3.2.4 Dendrimer Hosts: Non-Polar Interactions with the Dendrimer Surface Group 72

3.2.5 Dendrimer Hosts: Polar Interactions with the Dendrimer Surface Groups 73

3.3 Covalently Bound Drug-Dendrimer Conjugates 76

3.3.1 Self-Immolative Systems 79

3.4 Dendrimers as Gene Transfer Reagents 81

Chapter 4 Dendrimer Drugs 90

4.2 Antiviral Dendrimers 91

4.3 Antibacterial Dendrimers 97

4.4 Dendrimers in Antitumour Therapy 103

4.5 Dendrimers in Therapy of Other Diseases 106

4.6 Dendrimer-Based Vaccines 107

4.7 Dendrimer Interactions with Proteins. Solublilisation of Protein Aggregates 119

Chapter 5 Dendrimers in Diagnostics 130

5.1 Contrast Agents based on Dendrimers 130

5.1.1 Dendrimer-based Contrast Agents for CT 131

5.1.2 Dendrimer-based Contrast Agents for MRI 132

5.1.3 Dendrimer-based Contrast Agents for Scintigraphy 133

5.2 Fluorescence Enhanced by Dendrimers 133

5.3 Dendrimers in Bioassays 135

5.3.1 Dendrimer-Enhanced Signal Generation 135

5.3.2 Dendrimers for Amplifying the Covalent Binding Capacity of Solid Phases 139

5.3.3 Microarray Application of Dendrimers 141

5.3.4 Fluorophore-Labelled Dendrimers for Visualisation Purposes 145

5.3.5 Using Dendrimers to Increase DNA Extraction Yields 147

5.3.6 Using Dendrimers for direct Detection of Live Bacteria 148

Chapter 6 Dendrimers as Biomimics 152

6.2 Dendrimers as Protein Mimics 153

6.3 Dendrimers as Artificial Enzymes 161

6.4 Dendrimers as Artificial Antigens, Cell Surfaces and Antibodies 167.

Notes:

Includes bibliographical references and index.

ISBN:

0854048529

OCLC:

69482727

Publisher Number:

9780854048526