Cell-cell interactions : methods and protocols / edited by Sean P. Colgan.

Format:

Book

Contributor:

Colgan, Sean P.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 341.

Methods in molecular biology, 1064-3745 ; v. 341

Language:

English

Subjects (All):

Cell interaction.

Cell junctions.

Epithelial cells.

Cell Communication--physiology.

Endothelial Cells--physiology.

Epithelial Cells--physiology.

Tight Junctions--physiology.

Medical Subjects:

Cell Communication--physiology.

Endothelial Cells--physiology.

Epithelial Cells--physiology.

Tight Junctions--physiology.

Physical Description:

xiii, 286 pages, 4 unnumbered pages of color plates : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2006]

Summary:

A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology??? series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Contents:

Blood-brain barrier and cell-cell interactions: methods for establishing in vitro models of the blood-brain barrier and transport measurements / Michael Aschner ... [et al.]

Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences / Geraldine Canny, Alexander Swidsinski, and Beth A. McCormick

The role of junctional adhesion molecules in interactions between vascular cells / Triantafyllos Chavakis and Valeria Orlova

Cell-cell interactions on solid matrices / Nancy A. Louis, Dionne Daniels, and Sean P. Colgan

Cell-cell interactions in the kidney: inducible expression of mutant G protein [alpha]-subunits in Madin-Darby canine kidney cells for studies of epithelial cell junction structure and function / Ernesto Sabath and Bradley M. Denker

Nucleotide metabolism and cell-cell interactions / Holger K. Eltzschig ... [et al.]

Analysis of mammalian sperm-egg membrane interactions during in vitro fertilization / Genevieve B. Wortzman, Allison J. Gardner, and Janice P. Evans

Collagen gel contraction assay / Peter Ngo ... [et al.]

Methods to assess tissue permeability / Juan C. Ibla and Joseph Khoury

Bacterial-bacterial cell interactions in biofilms: detection of polysaccharide intercellular adhesins by blotting and confocal microscopy / Kimberly K. Jefferson and Nuno Cerca

A biochemical method for tracking cholera toxin transport from plasma membrane to golgi and endoplasmic reticulum / Heidi E. De Luca and Wayne I. Lencer

Isolation and culture of murine heart and lung endothelial cells for in vitro model systems / Yaw-Chyn Lim and Francis W. Luscinskas

Expression cloning of signaling proteins regulated by cell adhesion / Michelle L. Matter and Joe W. Ramos

Assays for the calcium sensitivity of Desmosomes / Anita J. Merritt, Anthea Scothern, and Tanusree Bhattacharyya

Tight junctions and cell-cell interactions / Markus Utech, Matthias Brüwer, and Asma Nusrat

Simultaneous immunofluorescence detection of cadherin and [beta]-catenin in mouse tissue sections / Nobuko Obara

In vitro neutrophil transepithelial migration / Winston Y. Lee ... [et al.]

The blot rolling assay: a method for identifying adhesion molecules mediating binding under shear conditions / Robert Sackstein and Robert Fuhlbrigge

Cell-cell interaction in the transcellular biosynthesis of novel [omega]-3-derived lipid mediators / Nan Chiang and Charles N. Serhan

Isolation and analysis of lipid rafts in cell-cell interactions / Aimee Landry and Ramnik Xavier.

Notes:

Includes bibliographical references and index.

ISBN:

1588295230

OCLC:

62123271