Principles of gene manipulation and genomics / S. B. Primrose and R. M. Twyman.

Format:

Book

Author/Creator:

Primrose, S. B.

Contributor:

Twyman, Richard M.

Primrose, S. B.

Language:

English

Subjects (All):

Genetic engineering.

Genomics.

Gene mapping.

Nucleotide sequence.

Genetic Engineering.

Base Sequence.

Chromosome Mapping.

DNA, Recombinant.

Medical Subjects:

Genetic Engineering.

Base Sequence.

Chromosome Mapping.

DNA, Recombinant.

Genomics.

Physical Description:

xxii, 644 pages : illustrations ; 28 cm

Edition:

Seventh edition.

Place of Publication:

Malden, MA : Blackwell Pub., 2006.

Summary:

There has been rapid progress in recombinant DNA technology over the last few years, particularly in the area of large-scale biology (genomics and related disciplines). The increasing integration between gene manipulation and genomics is embraced in this book, Principles of Gene Manipulation and Genomics, which brings together for the first time the subjects covered by the best-selling books Principles of Gene Manipulation and Principles of Genome Analysis and Genomics. This important new text has been comprehensively revised, updated, and rewritten to encompass within one volume, basic and advanced gene-manipulation techniques, genome analysis, genomics, transcriptomics, proteomics, and metabolomics, as well as the applications of these fascinating technologies.

Contents:

Gene manipulation in the post-genomics era

Basic techniques

Cutting and joining DNA molecules

Basic biology of plasmid and phage vectors

Cosmids, phasmids and other advanced vectors

Gene cloning strategies

Sequencing genes and short stretches of DNA

Changing genes: site-directed mutagenesis and protein engineering

Bioinformatics

Cloning in bacteria other than Escherichia coli

Cloning in Saccharomyces cerevisiae and other fungi

Gene transfer to animal cells

Genetic manipulation of animals

Gene transfer to plants

Advanced transgenic technology

The organization and structure of genomes

Mapping and sequencing genomes

Comparative genomics

Large scale mutagenesis and interference

Analysis of the transcriptome

Proteomics I

Expression analysis and characterization of proteins

Proteomics II

Analysis of protein structures

Proteomics III

Protein interactions

Metabolomics and global biochemical networks

Applications of genomics: understanding the basis of polygenic disorders and identifying quantitative trait loci

Applications of recombinant DNA technology.

Notes:

Rev. ed. of: Principles of gene manipulation. 6th ed. 2001 and: Principles of genome analysis and genomics / Sandy B. Primrose, Richard M. Twyman. 3rd ed. 2003.

Includes bibliographical references (pages [547]-626) and index.

Related Work:

Primrose, S. B. Principles of gene manipulation.

Primrose, S. B. Principles of genome analysis and genomics.

ISBN:

1405135441

OCLC:

60776718