Gene mapping, discovery, and expression : methods and protocols / edited by Minou Bina.

Format:

Book

Contributor:

Bina, Minou.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 338.

Methods in molecular biology ; 338

Language:

English

Subjects (All):

Gene mapping--Methodology.

Gene mapping.

Gene mapping--Data processing.

Genetics--Technique.

Genetics.

Gene expression.

Chromosome Mapping--methods.

Databases, Nucleic Acid.

Gene Expression Profiling--methods.

Microarray Analysis--methods.

Medical Subjects:

Chromosome Mapping--methods.

Databases, Nucleic Acid.

Gene Expression Profiling--methods.

Microarray Analysis--methods.

Genre:

Laboratory manuals.

Physical Description:

xiii, 334 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2006]

Summary:

Completion of the sequence of the human genome and the development of high-throughput technologies have opened a new era of research in biology and medicine. In Gene Mapping, Discovery, and Expression: Methods and Protocols, a panel of researchers from around the world and from a variety of disciplines offer a unique blend of computational tools and experimental techniques for gene mapping, comparative genomics, and studies of the control of gene expression. On the computational side, for beginners in the field, the book describes web-based technologies to localize and map genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential "codes" in genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis and characterization of transcription factor binding elements, and methods for isolation and identification of proteins that interact with DNA. The protocols in the book follow the successful Methods in Molecular Biology series format, offering step-by-step instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls.

Cutting-edge and interdisciplinary, Gene Mapping, Discovery, and Expression: Methods and Protocols offers a collection of DNA-sequence-based technologies for gene discovery, retrieval of gene expression data, discovery of regulatory elements, and discovery of transcription factors that interact with DNA.

Contents:

Use of genome browsers to locate your favorite genes / Minou Bina

Methods for identifying and mapping recent segmental and gene duplications in eukaryotic genomes / Razi Khaja ... [et al.]

Identification and mapping of paralogous genes on a known genomic DNA sequence / Minou Bina

Quantitative DNA fiber mapping in genome research and construction of physical maps / Heinz-Ulrich G. Weier and Lisa W. Chu

PRINS for mapping single copy genes / Avirachan T. Tharapel and Stephen S. Wachtel

Vista family of computational tools for comparative analysis of DNA sequences and whole genomes / Inna Dubchak and Dmitriy V. Ryaboy

Computational prediction of cis-regulatory modules from multispecies alignments using Galaxy, table browser, and GALA / Laura Elnitski, David King, and Ross C. Hardison

Comparative promoter analysis in vertebrate genomes with the CORG workbench / Christoph Dieterich and Martin Vingron

cis-regulatory region analysis using BEARR / Vinsensius Berlian Vega

A database of 9-mers from promoter regions of human protein-coding genes / Minou Bina, Phillip Wyss, and Syed Rehan Shah

A program toolkit for the analysis of regulatory regions of genes / Phillip Wyss, Sheryl A. Lazarus, and Minou Bina

Analysis of allele-specific gene expression / Julian C. Knight

Construction of micro-rna containing vectors for expression in mammalian cells / Yoko Fukuda, Hiroaki Kawasaki, and Kazunari Taira

Mining microarray data at NCBI's gene expression omnibus (GEO) / Tanya Barrett and Ron Edgar

The Stanford microarray database: a user's guide / Jeremy Gollub, Catherine A. Ball, and Gavin Sherlock

Detecting nucleosome ladders on unique DNA sequences in mouse liver nuclei / Tomara J. Fleury, Alfred Cioffi, and Arnold Stein

DNA methyltransferase probing of DNA-protein interactions / Scott A. Hoose and Michael P. Kladde

Protein binding microarrays (PBMS) for rapid, high-throughput characterization of the sequence specificities of DNA binding proteins / Michael F. Berger and Martha L. Bulyk

Quantitative profiling of protein-DNA binding on microarrays / Jiannis Ragoussis, Simon Field, and Irina A. Udalova

Analysis of protein-DNA binding by streptavidin-agarose pulldown / Kenneth K. Wu

Isolation and mass spectrometry of specific DNA-binding proteins / Mariana Yaneva and Paul Tempst

Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads / Patrick Rodriguez ... [et al.].

Notes:

Includes bibliographical references and index.

ISBN:

1588295753

OCLC:

61404526

Publisher Number:

9781588295750