Molecular and cellular biophysics / Meyer B. Jackson.

Format:

Book

Author/Creator:

Jackson, Meyer B.

Contributor:

Emma Louise McClellan Fund.

Language:

English

Subjects (All):

Biophysics.

Medical Subjects:

Biophysics.

Physical Description:

xiii, 512 pages : illustrations ; 25 cm

Place of Publication:

Cambridge : Cambridge University Press, 2006.

Summary:

This book provides advanced undergraduate and beginning graduate students with a foundation in the basic concepts of molecular and cellular biophysics. Students who have taken physical chemistry and calculus courses will find this book an accessible and valuable aid in learning how these concepts can be used in biological research. The text provides a rigorous treatment of the fundamental theories in biophysics and illustrates their application with examples. Conformational transitions of proteins are studied first using thermodynamics, and subsequently with kinetics. Allosteric theory is developed as the synthesis of conformational transitions and association reactions. Basic ideas of thermodynamics and kinetics are applied to topics such as protein folding, enzyme catalysis, and ion channel permeation. These concepts are then used as the building blocks in a treatment of membrane excitability. Through these examples, students will gain an understanding of the general importance and broad applicability of biophysical principles to biological problems.

Contents:

Chapter 1 Global transitions in proteins 1

1.1 Defining a global state 2

1.2 Equilibrium between two global states 4

1.3 Global transitions induced by temperature 5

1.4 Lysozyme unfolding 7

1.5 Steepness and enthalpy 9

1.6 Cooperativity and thermal transitions 11

1.7 Transitions induced by other variables 12

1.8 Transitions induced by voltage 14

1.9 The voltage sensor of voltage-gated channels 17

1.10 Gating current 18

1.11 Cooperativity and voltage-induced transitions 19

1.12 Compliance of a global state 21

Chapter 2 Molecular forces in biological structures 25

2.1 The Coulomb potential 25

2.2 Electrostatic self-energy 27

2.3 Image forces 29

2.4 Charge-dipole interactions 31

2.5 Induced dipoles 32

2.6 Cation-[pi] interactions 33

2.7 Dispersion forces 35

2.8 Hydrophobic forces 36

2.9 Hydration forces 39

2.10 Hydrogen bonds 39

2.11 Steric repulsions 43

2.12 Bond flexing and harmonic potentials 44

2.13 Stabilizing forces in proteins 46

2.14 Protein force fields 50

2.15 Stabilizing forces in nucleic acids 52

2.16 Lipid bilayers and membrane proteins 53

Chapter 3 Conformations of macromolecules 56

3.1 n-Butane 56

3.2 Configurational partition functions and polymer chains 58

3.3 Statistics of random coils 60

3.4 Effective segment length 62

3.5 Nonideal polymer chains and theta solvents 63

3.6 Probability distributions 65

3.7 Loop formation 66

3.8 Stretching a random coil 67

3.9 When do molecules act like random coils? 68

3.10 Backbone rotations in proteins: secondary structure 68

3.11 The entropy of protein denaturation 71

3.12 The helix-coil transition 73

3.13 Mathematical analysis of the helix-coil transition 74

3.14 Results of helix-coil theory 78

3.15 Helical propensities 80

3.16 Protein folding 82

3.17 Cooperativity in protein folding 86

Chapter 4 Molecular associations 89

4.1 Association equilibrium in solution 89

4.2 Cooperativity 91

4.2.1 Concerted binding 91

4.2.2 Sequential binding 93

4.2.3 Nearest neighbor interactions 94

4.3 Thermodynamics of associations 94

4.4 Contact formation 95

4.5 Statistical mechanics of association 96

4.6 Translational free energy 98

4.7 Rotational free energy 101

4.8 Vibrational free energy 102

4.9 Solvation effects 105

4.10 Configurational free energy 106

4.11 Protein association in membranes - reduction of dimensionality 107

4.12 Binding to membranes 108

Chapter 5 Allosteric interactions 111

5.1 The allosteric transition 112

5.2 The simplest case: one binding site and one allosteric transition 112

5.3 Binding and response 115

5.4 Energy balance in the one-site model 116

5.5 G-protein coupled receptors 117

5.6 Binding site interactions 121

5.7 The Monod-Wyman-Changeux (MWC) model 123

5.8 Hemoglobin 126

5.9 Energetics of the MWC model 127

5.10 Macroscopic and microscopic additivity 128

5.11 Phosphofructokinase 130

5.12 Ligand-gated channels 132

5.13 Subunit-subunit interactions: the Koshland-Nemethy-Filmer (KNF) model 134

5.14 The Szabo-Karplus (SK) model 137

Chapter 6 Diffusion and Brownian motion 142

6.1 Macroscopic diffusion: Fick's laws 142

6.2 Solving the diffusion equation 143

6.2.1 One-dimensional diffusion from a point 144

6.2.2 Three-dimensional diffusion from a point 146

6.2.3 Diffusion across an interface 146

6.2.4 Diffusion with boundary conditions 148

6.3 Diffusion at steady state 150

6.3.1 A long pipe 151

6.3.2 A small hole 152

6.3.3 A porous membrane 153

6.4 Microscopic diffusion - random walks 154

6.5 Random walks and the Gaussian distribution 156

6.6 The diffusion equation from microscopic theory 159

6.7 Friction 160

6.8 Stokes' law 162

6.9 Diffusion constants of macromolecules 163

6.10 Lateral diffusion in membranes 164

Chapter 7 Fundamental rate processes 167

7.1 Exponential relaxations 167

7.2 Activation energies 169

7.3 The reaction coordinate and detailed balance 170

7.4 Linear free energy relations 172

7.5 Voltage-dependent rate constants 175

7.6 The Marcus free energy relation 177

7.7 Eyring theory 179

7.8 Diffusion over a barrier - Kramers' theory 180

7.9 Single-channel kinetics 183

7.10 The reaction coordinate for a global transition 186

Chapter 8 Association kinetics 194

8.1 Bimolecular association 194

8.2 Small perturbations 195

8.3 Diffusion-limited association 197

8.4 Diffusion-limited dissociation 200

8.5 Site binding 201

8.6 Protein-ligand association rates 203

8.6.1 Evolution of speed 205

8.6.2 Acetylcholinesterase 205

8.6.3 Horseradish peroxidase 206

8.7 Proton transfer 207

8.8 Binding to membrane receptors 208

8.9 Reduction in dimensionality 212

8.10 Binding to DNA 214

Chapter 9 Multi-state kinetics 216

9.1 The three-state model 216

9.2 Initial conditions 219

9.3 Separation of timescales 220

9.4 General solution to multi-state systems 221

9.5 The three-state model in matrix notation 225

9.6 Stationarity, conservation, and detailed balance 226

9.7 Single-channel kinetics: the three-state model 229

9.8 Separation of timescales in single channels: burst analysis 232

9.9 General treatment of single-channel kinetics: state counting 235

9.10 Relation between single-channel and macroscopic kinetics 236

9.11 Loss of stationarity, conservation, and detailed balance 237

9.12 Single-channel correlations: pathway counting 240

9.13 Multisubunit kinetics 242

9.14 Random walks and "stretched kinetics" 244

Chapter 10 Enzyme catalysis 248

10.1 Basic mechanisms - serine proteases 248

10.2 Michaelis-Menten kinetics 251

10.3 Steady-state approximations 254

10.4 Pre-steady-state kinetics 256

10.5 Allosteric enzymes 257

10.6 Utilization of binding energy 258

10.7 Kramers' rate theory and catalysis 259

10.8 Proximity and translational entropy 260

10.9 Rotational entropy 263

10.10 Reducing E[superscript dagger]: transition state complementarity 264

10.11 Friction in an enzyme-substrate complex 267

10.12 General-acid-base catalysis and Bronsted slopes 268

10.13 Acid-base catalysis in [beta]-galactosidase 270

10.14 Catalysis in serine proteases and strong H-bonds 272

10.15 Marcus' theory and proton transfer in carbonic anhydrase 273

Chapter 11 Ions and counterions 276

11.1 The Poisson-Boltzmann equation and the Debye length 277

11.2 Activity coefficient of an ion 279

11.3 Ionization of proteins 283

11.4 Gouy-Chapman theory and membrane surface charge 285

11.5 Stern's improvements of Gouy-Chapman theory 288

11.6 Surface charge and channel conductance 291

11.7 Surface charge and voltage gating 293

11.8 Electrophoretic mobility 294

11.9 Polyelectrolyte solutions I. Debye-Huckel screening 297

11.10 Polyelectrolyte solutions II.

Counterion-condensation 300

11.11 DNA melting 302

Chapter 12 Fluctuations 307

12.1 Deviations from the mean 307

12.2 Number fluctuations and the Poisson distribution 309

12.3 The statistics of light detection by the eye 311

12.4 Equipartition of energy 313

12.5 Energy fluctuations in a macromolecule 315

12.6 Fluctuations in protein ionization 317

12.7 Fluctuations in a two-state system 319

12.8 Single-channel current 320

12.9 The correlation function of a two-state system 322

12.10 The Wiener-Khintchine theorem 324

12.11 Channel noise 327

12.12 Circuit noise 329

12.13 Fluorescence correlation spectroscopy 332

12.14 Friction and the fluctuation-dissipation theorem 336

Chapter 13 Ion permeation and membrane potential 339

13.1 Nernst potentials 339

13.2 Donnan potentials 341

13.3 Membrane potentials of cells 343

13.3.1 Neurons 345

13.3.2 Vertebrate skeletal muscle 345

13.4 A membrane permeable to Na[superscript +] and K[superscript +] 347

13.5 Membrane potentials of neurons again 350

13.6 The Ussing flux ratio and active transport 351

13.7 The Goldman-Hodgkin-Katz voltage equation 352

13.8 Membrane pumps and potentials 354

13.9 Transporters and potentials 355

13.10 The Goldman-Hodgkin-Katz current equation 357

13.11 Divalent ions 360

13.12 Surface charge and membrane potentials 361

13.13 Rate theory and membrane potentials 362

Chapter 14 Ion permeation and channel structure 367

14.1 Permeation without channels 367

14.2 The Ohmic channel 370

14.3 Energy barriers and channel properties 371

14.4 Eisenman selectivity sequences 374

14.5 Forces inside an ion channel 376

14.6 Gramicidin A 378

14.7 Rate theory for multibarrier channels 380

14.8 Single-ion channels 384

14.9 Single-file channels 390

14.10 The KcsA channel 394

Chapter 15 Cable theory 400

15.1 Current through membranes and cytoplasm 401

15.2 The cable equation 403

15.3 Steady state in a finite cable 406

15.4 Voltage steps in a finite cable 408

15.5 Current steps in a finite cable 411

15.6 Branches and equivalent cylinder representations 412

15.6.1 Steady state 413

15.6.2 Time constants 415

15.7 Cable analysis of a neuron 418

15.8 Synaptic integration in dendrites: analytical models 422

15.8.1 Impulse responses 423

15.8.2 Realistic synaptic inputs 425

15.9 Compartmental models and cable theory 428

15.10 Synaptic integration in dendrites: compartmental models 430

Chapter 16 Action potentials 434

16.1 The action potential 434

16.2 The voltage clamp and the properties of Na[superscript +] and K[superscript +] channels 439

16.3 The Hodgkin-Huxley equations 442

16.4 Current-voltage curves and thresholds 447

16.5 Propagation 450

16.6 Myelin 453

16.7 Axon geometry and conduction 455

16.8 Channel diversity 457

16.9 Repetitive activity and the A-current 458

16.10 Oscillations 461

16.11 Dendritic integration 466

Appendix 1 Expansions and series 470

A1.1 Taylor series 470

A1.2 The binomial expansion 471

A1.3 Geometric series 471

Appendix 2 Matrix algebra 472

A2.1 Linear transforms 472

A2.2 Determinants 473

A2.3 Eigenvalues, eigenvectors, and diagonalization 474

Appendix 3 Fourier analysis 477

Appendix 4 Gaussian integrals 481

Appendix 5 Hyperbolic functions 483

Appendix 6 Polar and spherical coordinates 484.

Notes:

Includes bibliographical references (pages [486]-503) and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Emma Louise McClellan Fund.

ISBN:

052162441X

0521624703

OCLC:

61757063

Publisher Number:

9780521624411

9780521624701