Epidermal growth factor : methods and protocols / edited by Tarun B. Patel, Paul J. Bertics.

Format:

Book

Contributor:

Patel, Tarun B., 1953-

Bertics, Paul J.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 327.

Methods in molecular biology ; 327

Language:

English

Subjects (All):

Epidermal growth factor--Laboratory manuals.

Epidermal growth factor.

Epidermal Growth Factor--analysis.

Epidermal Growth Factor--physiology.

ErbB Receptors--analysis.

ErbB Receptors--physiology.

Medical Subjects:

Epidermal Growth Factor--analysis.

Epidermal Growth Factor--physiology.

ErbB Receptors--analysis.

ErbB Receptors--physiology.

Genre:

Laboratory manuals.

Physical Description:

xi, 208 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2006]

Summary:

Epidermal growth factor (EGF) and related molecules regulate a wide array of biological events, ranging from normal growth and development to such pathological states as cancer and cardiovascular diseases. In Epidermal Growth Factor: Methods and Protocols, expert researchers active in the field present their best methods to dissect the mechanisms that control growth factors and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and highly practical, Epidermal Growth Factor: Methods and Protocols offers investigators an array of powerful and proven tools to unravel the workings of epidermal growth factor, as well as develop new strategies for the successful use of this important developmental and therapeutic target.

Contents:

A historical perspective of EGF receptor and related systems / Francis Edwin ... [et al.]

Purification and assay of kinase-active EGF receptor from mammalian cells by immunoaffinity chromatography / Gregory J. Wiepz ... [et al.]

Soluble epidermal growth factor receptor acridinium-linked immunosorbent assay / Jacqueline M. Lafky, Andre T. Baron, and Nita J. Maihle

Analysis of EGF receptor interactions with the [alpha] subunit of the stimulatory GTP binding protein of adenylyl cyclase, Gs / Deepti Chaturvedi ... [et al.]

Regulator of epidermal growth factor signaling: sprouty / Esther Sook Miin Wong and Graeme R. Guy

Dissecting the epidermal growth factor receptor signal transactivation pathway / Oliver M. Fischer, Stefan Hart, and Axel Ullrich

A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor / Umut Sahin ... [et al.]

In vitro and in vivo assays of monoubiquitination of receptor tyrosine kinases / Manachem Katz, Yaron Mosesson, and Yosef Yarden

Assays to monitor degradation of the EGF receptor / Mirko H.H. Schmidt and Ivan Dikic

Assessment of degradation and ubiquitination of CXCR4, a GPCR regulated by EGFR family members / Adriano Marchese

Epithelial cell migration in response to epidermal growth factor / Reema Zeineldin and Laurie G. Hudson

Motility signaled from EGF receptor and related systems / Alan Wells ... [et al.]

Methods for determining the proliferation of cells in response to EGFR ligands / Gregory J. Wiepz ... [et al.]

Clinical advancement of EGFR inhibitors in cancer therapy / Prakash Chinnaiyan and Paul M. Harari.

Notes:

Includes bibliographical references and index.

ISBN:

1588294218

159745012X

OCLC:

60669033