Nuclear reprogramming : methods and protocols / edited by Steve Pells.

Format:

Book

Contributor:

Pells, Steve.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 325.

Methods in molecular biology, 1064-3745 ; 325

Language:

English

Subjects (All):

Cell nuclei--Transplantation--Laboratory methods.

Cell nuclei.

Cell Nucleus--physiology.

Cell Fusion--methods.

Transplantation--methods.

Cell nuclei--Transplantation.

Medical Subjects:

Cell Nucleus--physiology.

Cell Fusion--methods.

Transplantation--methods.

Physical Description:

xiv, 333 pages : illustrations (some color) ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2006]

Contents:

Nuclear transplantation in xenopus / John B. Gurdon

Nuclear transfer in sheep / William A. Ritchie

Protocols for nuclear transfer in mice / Shaorong Gao

Isolation of stromal stem cells from human adipose tissue / Andrew C. Boquest ... [et al.]

Nuclear reprogramming by cell fusion / Robert H. Broyles ... [et al.]

Polyethylene glycol-mediated cell fusion / Jian Yang and Ming Hong Shen

Epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells / Masako Tada and Takashi Tada

Quantification of cell fusion by flow cytometry / Stephen Sullivan ... [et al.]

Modulation of cell fate using nuclear and cytoplasmic extracts / Anne-Mari Håkelien, Kristine G. Gaustad, and Philippe Collas

Transgenic systems in nuclear reprogramming / Megan Munsie, Peter Mountford, and Jennifer Nichols

Using immunofluorescence to observe methylation changes in mammalian preimplantation embryos / Fátima Santos and Wendy Dean

Observing s-phase dynamics of histone modifications with fluorescently labeled antibodies / Rong Wu, Anna V. Terry, and David M. Gilbert

Quantitative analysis of telomerase activity and telomere length in domestic animal clones / Dean H. Betts ... [et al.]

Pluripotency: capacity for in vitro differentiation of undifferentiated embryonic stem cells / Cornelia Wiese ... [et al.]

Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens / Bruce A. Fenderson ... [et al.]

Analysis of the nucleolar compartment of the nucleus as an indicator of nuclear reprogramming after nuclear transfer / Jacques-E. Fléchon

Methylation-sensitive polymerase chain reaction / Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young

Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis / Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang

Chromatin immunoprecipitation assay for mammalian tissues / Fiona B. Turner, Wang L. Cheung, and Peter Cheung

Histone modifications and transcription factor binding on chromatin: CHIP/PCR assays / Jaejoon Won and Tae Kook Kim

In vivo genomic footprinting using LM-PCR methods / Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer

Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease / Pascal Lefevre and Constanze Bonifer.

Notes:

Includes bibliographical references and index.

ISBN:

1588293793

1597450057

OCLC:

61121123

Publisher Number:

9781588293794