G protein-coupled receptors : structure, function, and ligand screening / edited by Tatsuya Haga, Shigeki Takeda.

Format:

Book

Contributor:

Haga, Tatsuya.

Takeda, Shigeki.

Series:

Methods in signal transduction ; 6.

Methods in signal transduction ; 6

Language:

English

Subjects (All):

G proteins--Receptors.

G proteins.

Receptors, G-Protein-Coupled.

GTP-Binding Proteins.

Medical Subjects:

Receptors, G-Protein-Coupled.

GTP-Binding Proteins.

Genre:

Laboratory manuals.

Physical Description:

315 pages, 4 unnumbered pages of plates : illustrations (partly color) ; 24 cm.

Place of Publication:

Boca Raton : CRC Press/Taylor & Francis, 2006.

Summary:

G Protein-Coupled Receptors: Structure, Function, and Ligand Screening is a not a revision but rather a continuation of G Protein-Coupled Receptors, published in 1999. This newer work covers techniques in the field of GPCRs, which either were not treated in the original or have since been developed. In particular, it places significant emphasis on the crystallography of rhodopsin, which was first discovered in 2000, and is still the only GPCR that has had its atomic structure revealed.

G protein-coupled receptors (GPCRs) constitute one of the largest superfamilies of proteins. They are valued for their role as primary cell sensors and for their potential as drug targets. The molecular function of GPCRs is to recognize ligands and then activate G proteins. Those receptors with unidentified endogenous ligands are referred to as orphan GPCRs.

Written by those experts who pioneered the very methods discussed, G Protein-Coupled Receptors: Structure, Function, and Ligand Screening reviews the state of research on orphan GPCRs. Ultimately, the work is intended to elucidate the structural basis for the GPCR-ligand interaction and the molecular mechanisms underlying the function and regulation of GPCRs. This will allow researchers to utilize their interactions with ligands for drug design.

Contents:

Part I Screening of Ligands for GPCRs

Chapter 1 Screening of Endogenous Ligands for Orphan GPCRs / Masaaki Mori, Shuji Hinuma, Testuya Ohtaki, Masahiko Fujino 3

Chapter 2 Screening of Ligands for Human GPCRs by the Use of Receptor-G[alpha] Fusion Proteins / Shigeki Takeda, Hinako Suga, Tatsuya Haga 37

Chapter 3 Screening and Identification of Ghrelin, an Endogenous Ligand for GHS-R / Masayasu Kojima, Kenji Kangawa 67

Chapter 4 Ligand Screening of Olfactory Receptors / Kazushige Touhara, Sayako Katada, Takao Nakagawa, Yuki Oka 85

Part II Functions and Regulations of GPCRs

Chapter 5 Generation and Phenotypical Analysis of Muscarinic Acetylcholine Receptor Knockout Mice / Jurgen Wess, Dinesh Gautam, Sung-Jun Han, Jongrye Jeon, Cuiling Li, Chuxia Deng 113

Chapter 6 Systematic Mutagenesis of M[subscript 1] Muscarinic Acetylcholine Receptors / Edward C. Hulme 137

Chapter 7 Analysis of the Regulation of Muscarinic Acetylcholine Receptors / Chris J. van Koppen 179

Chapter 8 Single-Molecule Analysis of Chemotactic Signaling Mediated by cAMP Receptor on Living Cells / Masahiro Ueda, Yukihiro Miyanaga, Toshio Yanagida 197

Chapter 9 Oligomerization of G Protein-Coupled Purinergic Receptors / Hiroyasu Nakata, Kazuaki Yoshioka, Toshio Kamiya 219

Part III Tertiary Structure of GPCRs and Their Ligands

Chapter 10 Methods and Results in X-Ray Crystallography of Bovine Rhodopsin / Tetsuji Okada, Rumi Tsujimoto, Miho Muraoka, Chihiro Funamoto 243

Chapter 11 Determination of Steric Structure of Muscarinic Ligands Bound to Muscarinic Acetylcholine Receptors: Approaches by TRNOE (Transferred Nuclear Overhauser Effect) / Hiroyasu Furukawa, Toshiyuki Hamada, Hiroshi Hirota, Masaji Ishiguro, Tatsuya Haga 261

Chapter 12 Modeling of G Protein-Coupled Receptors for Drug Design / Masaji Ishiguro 283.

Notes:

Includes bibliographical references and index.

ISBN:

0849327717

OCLC:

58051444