Human papillomaviruses : methods and protocols / edited by Clare Davy, John Doorbar.

Format:

Book

Contributor:

Davy, Clare.

Doorbar, John.

Series:

Methods in molecular medicine ; 119.

Methods in molecular medicine ; 119

Language:

English

Subjects (All):

Papillomavirus diseases--Laboratory manuals.

Papillomavirus diseases.

Papillomaviruses--Laboratory manuals.

Papillomaviruses.

Alphapapillomavirus--genetics.

Alphapapillomavirus--pathogenicity.

Uterine Cervical Neoplasms--virology.

Medical Subjects:

Alphapapillomavirus--genetics.

Alphapapillomavirus--pathogenicity.

Uterine Cervical Neoplasms--virology.

Genre:

Laboratory manuals.

Physical Description:

xv, 496 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2005]

Summary:

Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine??? series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Contents:

Identification of new papillomavirus types / Ethel-Michele de Villiers, Corinna Whitley, and Karin Gunst

Identification of HPV variants / John Cason, Jon Bible, and Christine Mant

Histochemical analysis of cutaneous HPV-associated lesions / Kiyofumi Egawa

Histological analysis of cervical intraepithelial neoplasia / Michael Babawale ... [et al.]

Detection of papillomavirus proteins and DNA in paraffin-embedded tissue sections / Woei Ling Peh and John Doorbar

Detection and quantitation of HPV gene expression using real-time PCR / Rashmi Seth ... [et al.]

Analysis of P16INK4A and integrated HPV genomes as progression markers / Svetlana Vinokurova, Nicolas Wentzensen, and Magnus von Knebel Doeberitz

Use of biomarkers in the evaluation of CIN grade and progression of early CIN / Jan P.A. Baak and Arnold-Jan Kruse

HPV DNA detection and typing in cervical scrapes / Peter J. F. Snijders ... [et al.]

HPV DNA detection and typing in inapparent cutaneous infections and premalignant lesions / Maurits de Koning ... [et al.]

Establishing HPV-containing keratinocyte cell lines from tissue biopsies / Margaret Anne Stanley

Using an immortalized cell line to study the HPV lifecycle in organotypic "raft" cultures / Paul F. Lambert ... [et al.]

Differentiation of HPV-containing cells using organotypic "raft" culture or methylcellulose / Regina Wilson and Laimonis A. Laimins

Propagation of infectious, high-risk HPV in organotypic "raft" culture / Margaret E. McLaughlin-Drubin and Craig Meyers

Retrovirus-mediated gene transfer to analyze HPV gene regulation and protein functions in organotypic "raft" cultures / N. Sanjib Banerjee, Louise T. Chow, and Thomas R. Broker

The HPV xenograft severe combined immunodeficiency (scid) mouse model / William Bonnez

The cottontail rabbit papillomavirus model of high-risk HPV-induced disease / Janet L. Brandsma

Studying the HPV lifecycle in 3A trophoblasts and resulting pathophysiology / Yong Liu, Hong You, and Paul L. Hermonat

Replication and encapsidation of papillomaviruses in saccharomyces cerevisiae / Peter C. Angeletti

Analysis of the regulation of viral transcription / Bernd Gloss, Mina Kalantari, and Hans-Ulrich Bernard

Analysis of HPV transcription by RPA / Jason M. Bodily and Craig Meyers

Analysis of regulatory motifs within HPV transcripts / Sarah A. Cumming and Sheila V. Graham

Detection of HPV transcripts by nested RT-PCR / Christine Mant, Barbara Kell, and John Cason

Analysis of HPV DNA replication using transient transfection and cell-free assays / Bing Yuan Lin, Thomas R. Broker, and Louise T. Chow

Detection and quantitation of HPV DNA replication by southern blotting and real-time PCR / Iain M. Morgan and Ewan R. Taylor

Analysis of E7/RB associations / Sandra Caldeira, Wen Dong, and Massimo Tommasino

Transformation assays for HPV oncoproteins / Paola Massimi and Lawrence Banks

Analysis of adeno-associated virus and hpv interaction / Paul L. Hermonat ... [et al.]

In vitro assays of substrate degradation induced by high-risk HPV E6 oncoproteins / Miranda Thomas and Lawrence Banks

Measuring the induction or inhibition of apoptosis by HPV proteins / Anna M. Kowalczyk ... [et al.]

Codon optimization of papillomavirus genes / Martin Muller

Generation of HPV pseudovirions using transfection and their use in neutralization assays / Christopher B. Buck ... [et al.]

Generation and application of HPV pseudovirions using vaccinia virus / Martin Sapp and Hans-Christoph Selinka.

Notes:

Includes bibliographical references and index.

ISBN:

1588293734

OCLC:

58452496