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Calcium signaling protocols / edited by David G. Lambert.
Holman Biotech Commons QH506 .M45 v.312 2006
Available
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) ; v. 312.
- Methods in molecular biology, 1064-3745 ; v. 312
- Language:
- English
- Subjects (All):
- Calcium--Laboratory manuals.
- Calcium.
- Calcium--Research--Methodology.
- Calcium channels--Research--Methodology.
- Calcium channels.
- Cellular signal transduction--Research--Methodology.
- Cellular signal transduction.
- Calcium--pharmacokinetics.
- Calcium Channels--pharmacokinetics.
- Calcium Signaling--physiology.
- Signal Transduction.
- Spectrometry, Fluorescence--methods.
- Research.
- Methodology.
- Medical Subjects:
- Calcium--pharmacokinetics.
- Calcium Channels--pharmacokinetics.
- Calcium Signaling--physiology.
- Signal Transduction.
- Spectrometry, Fluorescence--methods.
- Genre:
- Laboratory manuals.
- Physical Description:
- x, 359 pages : illustrations ; 24 cm.
- Edition:
- Second edition.
- Place of Publication:
- Totowa, N.J. : Humana Press, [2006]
- Summary:
- This extensively updated and expanded second edition contains new chapters on the elctrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluormetric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many dacets of Ca2+ signaling. The protocols follow the successful Methods in Molecular Biology??? series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
- Contents:
- Fluorescent measurement of [Ca2+]c: basic practical considerations / Alec W.M. Simpson
- Measurement of [Ca2+]i in whole cell suspensions using fura-2 / Robert A. Hirst ... [et al.]
- Measurement of [Ca2+]i in cell suspensions using indo-1 / Adriaan Nelemans
- Confocal microscopy: theory and applications for cellular signaling / Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars
- Single-cell and subcellular measurement of intracellular Ca2+ concentration / Anthony J. Morgan and Andrew P. Thomas
- Ratiometric Ca2+ measurements using the FlexStation scanning fluorometer / Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty
- Measuring Ca2+ changes in multiwell format using the Fluorometric Imaging Plate Reader / Ian C.B. Marshall, Davina E. Owen, and Shawn McNulty
- Measurement of Ca2+ entry using 45Ca2+ / Mercedes Villarroya ... [et al.]
- Measurement of [3H]PN200-110 and [125I]
- conotoxin MVIIA binding / Kazuyoshi Hirota and David G. Lambert
- Whole-cell patch clamp recording of voltage-sensitive Ca2+ channel currents: heterologous expression systems and dissociated brain neurons / Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani
- Measurement of phospholipase C by monitoring inositol phosphates using [3H]inositol-labeling protocols in permeabilized cells / Alison Skippen, Philip Swigart, and Shamshad Cockcroft
- Measurement of inositol(1,4,5)triphosphate using a stereospecific radioreceptor mass assay / Darren Smart
- Measurement of calcium fluxes in permeabilized cells using a 45Ca2+ uptake and release assay / Robert A. Wilcox
- Microinjection of myo-inositol(1,4,5)trisphosphate and other calcium-mobilizing agents into intact adherent cells / Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann
- Measurement of free [Ca2+] changes in agonist-sensitive internal stores using compartmentalized fluorescent indicators / Aldebaran M. Hofer
- Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry / Hideo Kanaide
- Measurement of calcium and movement in heart cells / Leong L. Ng and Paulene A. Quinn
- Simultaneous analysis of intracellular pH and Ca2+ from cell populations / Raul Martinez-Zaguilan ... [et al.]
- Measurement of cytosolic-free Ca2+ in plant tissue / Martin R. McAinsh and Carl K.-Y. Ng
- Assay and purification of calmodulin-dependent protein kinase / Rajendra K. Sharma
- Assay and purification of calmodulin-dependent cyclic nucleotide phosphodiesterase and isozyme separation / Rajendra K. Sharma
- Measurement of Ca2+-ATPase activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 1588294420
- OCLC:
- 57965822
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