Therapeutic proteins : methods and protocols / edited by C. Mark Smales and David C. James.

Format:

Book

Contributor:

Smales, C. Mark.

James, David C. (David Cameron), 1963-

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 308.

Methods in molecular biology ; 308

Language:

English

Subjects (All):

Protein drugs--Laboratory manuals.

Proteins--therapeutic use.

Proteins--chemical synthesis.

Proteins--isolation & purification.

Protein drugs.

Medical Subjects:

Proteins--therapeutic use.

Proteins--chemical synthesis.

Proteins--isolation & purification.

Genre:

Laboratory manuals.

Physical Description:

xiv, 482 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2005]

Summary:

World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology??? series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Contents:

Biopharmaceutical proteins / John R. Birch and Yemi Onakunle

Expression of antibody fragments by periplasmic secretion in Escherichia coli / Andrew G. Popplewell ... [et al.]

Secretory production of therapeutic proteins in Escherichia coli / Sang Yup Lee, Jong Hyun Choi, and Sang Jun Lee

Expression of recombinant LTB protein in marine vibrio VSP60 / Wang Yili and Si Lusheng

Heterologous gene expression in yeast / Lee J. Byrne, Kenneth J. O'Callaghan, and Mick F. Tuite

Pharmaceutical proteins from methylotrophic yeasts / Eric C. de Bruin ... [et al.]

Expression of human papillomavirus type 16 L1 in baculovirus expression systems / Zheng Jin, Si Lusheng, and Wang Yili

Large-scale transient expression of therapeutic proteins in mammalian cells / Sabine Geisse, Martin Jordan, and Florian M. Wurm

Site-specific integration for high-level protein production in mammalian cells / Bhaskar Thyagarajan and Michele P. Calos

Production of recombinant therapeutic proteins by mammalian cells in suspension culture / Lily Chu, Ilse Blumentals, and Gargi Maheshwari

Transgene control engineering in mammalian cells / Beat P. Kramer and Martin Fussenegger

Fusion to albumin as a means to slow the clearance of small therapeutic proteins / William P. Sheffield, Teresa R. McCurdy, and Varsha Bhakta

High throughput recovery of therapeutic proteins from the inclusion bodies of Escherichia coli / Amulya K. Panda

The isolation, solubilization, refolding, and chromatographic purification of recombinant human growth hormone from inclusion bodies of Escherichia coli cells / Amulya K. Panda, A. N. S. Eshwari, and Lalit C. Garg

Large-scale preparation of factor VIIa from human plasma / Teruhisa Nakashima and Kazuhiko Tomokiyo

Purification of clinical-grade monoclonal antibodies by chromatographic methods / Alberto L. Horenstein, Ilaria Durelli, and Fabio Malavasi

Virus elimination and validation / Nicola Boschetti and Anna Johnston

Virus removal by nanofiltration / Marina Korneyeva and Scott Rosenthal

Determining residual host cell antigen levels in purified recombinant proteins by slot blot and scanning laser densitometry / Aaron P. Miles, Daming Zhu, and Allan Saul

Principles of biopharmaceutical protein formulation / Scott P. Sellers and Yuh-Fun Maa

Solid-state protein formulation / Yuh-Fun Maa and Scott P. Sellers

Stabilization of therapeutic proteins by chemical and physical methods / Ken-ichi Izutsu

Extraction and characterization of vaccine antigens from water-in-oil adjuvant formulations / Aaron P. Miles and Allan Saul

Probing reversible self-association of therapeutic proteins by sedimentation velocity in the analytical ultracentrifuge / Bernardo Perez-Ramirez and John J. Steckert

Biological characterization of pegylated interferons / Lei Xie ... [et al.]

Characterization of interferon alpha-2B pegylated via carboxyalkylation / David C. Wylie ... [et al.]

Quantifying recombinant proteins and their degradation products using SDS-PAGE and scanning laser densitometry / Aaron P. Miles and Allan Saul.

Notes:

Includes bibliographical references and index.

ISBN:

1588293904

1592599222

OCLC:

56526381