Cell cycle control : mechanisms and protocols / edited by Tim Humphrey, Gavin Brooks.

Format:

Book

Contributor:

Humphrey, Tim (Tim Carter)

Brooks, Gavin.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 296.

Methods in molecular biology ; 296

Language:

English

Subjects (All):

Cell cycle--Laboratory manuals.

Cell cycle.

Cell Cycle--physiology.

Eukaryotic Cells--physiology.

Molecular Biology--methods.

Medical Subjects:

Cell Cycle--physiology.

Eukaryotic Cells--physiology.

Molecular Biology--methods.

Genre:

Laboratory manuals.

Physical Description:

xii, 402 pages : illustrations ; cm.

Place of Publication:

Totowa, N.J. : Humana Press, [2005]

Summary:

This collection of cutting-edge techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on well-defined cell cycle regulators-and those more recently defined-in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The book offers all cell researchers indispensable techniques essential to understanding how normal cells divide and how this is altered in disease.

Contents:

Cell cycle molecules and mechanisms of the budding and fission yeasts / Tim Humphrey and Amanda Pearce

The plant cell cycle / John H. Doonan

The C. elegans cell cycle / Sander van den Heuvel

Developmental control of growth and cell cycle progression in drosophila / Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio

The xenopus cell cycle / Anna Philpott and P. Renee Yew

The mammalian cell cycle / Jane Vera Harper and Gavin Brooks

Synchronization of cell populations in G1/S and G2/M phases of the cell cycle / Jane Vera Harper

Mapping origins of DNA replication in eukaryotes / Susan A. Gerbi

In situ assay for analyzing the chromatin binding of proteins in fission yeast / Stephen E. Kearsey ... [et al.]

Application of magnetic beads to purify cells transiently transfected with plasmids encoding short hairpin RNAs / Peter D. Adams

The cell cycle and virus infection / Stevan R. Emmett ... [et al.]

Methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies / Julie Welburn and Jane Endicott

E2F transcription factors and pRb pocket proteins in cell cycle progression / Ludger Hauck and Rüdiger von Harsdorf

Forkhead (FOX) transcription factors and the cell cycle / Katrina A. Bicknell

Measurement of geminin activity in xenopus egg extracts / Thomas J. McGarry

CDK-activating kinases / Stéphane Larochelle and Robert P. Fisher

Cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors / Gavin Brooks

Measurement of wee kinase activity / Paul R. Mueller and Walter F. Leise III

CDC25 dual-specificity protein phosphatases / Sorab N. Dalal and Melanie Volkening

Assaying cell cycle checkpoints / Carmela Palermo and Nancy C. Walworth

Polo-like kinase-1 / Marcel A.T.M. van Vugt and René H. Medema

The Ipl1/aurora kinase family / Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor

Purification of the Ndc80 kinetochore subcomplex from xenopus eggs / Mark L. McCleland and P. Todd Stukenberg.

Notes:

Includes bibliographical references and index.

ISBN:

1588291448

OCLC:

55008713